The Effects Of Desflurane And Sevoflurane On Nesfatin1 Levels In Laparoscopic Cholecystectomy

The groups were randomly and equally identified by drawing group names from an envelope prior to anesthesia induction. Heart rate (HR), mean systolic and diastolic arterial pressures (SAP, DAP, MAP), peripheral O2 saturation (SpO2) of the patients who were taken to the operation were monitorized. A 20-gauge (G) branule was used to enable intravenous access, and 0.9% sodium chloride (5-10 mL/kg/h) was infused. To induce anesthesia, the patients were given 1 mg of lidocaine (Aritmal, 2%, Osel), 1 µg/kg of remifentanil (Ultiva, 5 mg, GlaxoSmithKline), 8 mg/kg of thiopental sodium, and 0.6 mg/kg of rocuronium bromide intravenously. After a three-min preoxygenation with 100% O2 administration through a face mask, orotracheal intubation was performed when sufficient muscle relaxation was observed. Then, patients were ventilated with a Drager anesthesia instrument (Luebeck, Germany), with tidal volume set to 10 mL/kg and frequency set to 12/min. Soda lime (Sorbo-lime, Berkim, Turkey) was used as a CO2 absorbant. For anesthesia maintenance, patients were randomly assigned to two groups. Patients in the first group received inhalation of 6 % desflurane in 60% O2, 40% air (Forane, Abbott Lab., England). Patients in the second group received 2% sevoflurane in 60% O2, 40% air. Patients in both groups received remifentanil infusion (0.25 µg/kg/min) to maintain anesthesia. At the end of the surgery, all patients received 0.5 mg atropine and 1.5 mg neostigmine for decurarization. In all groups, patients received 0.5 mg atropine when heart rate was <40; 10 mg of ephedrine when MAP was <50, and the infusion dose was lowered. Patients received intravenous tramadol (1 mg/kg) and methochloropropamide (10 mg) 30 min before the end of surgery. Hemodynamic and respiratory parameters (SAP, DAP, MAP, HR, SpO2, etCO2) were recorded before induction, after induction, after entubation, and during extubation. Blood samples were collected before induction (T1), and after extubation when aldrate score was 10 (T2). Blood samples were collected into aprotinin and EDTA-containing tubes. Samples were centrifuged at 3,000 rpm, and plasma samples were aliquoted in polypropylene tubes, and stored at -80 C until further analysis. Five cc of venous blood samples were used to analyze nesfatin-1 levels, by using a commercial ELISA kit according to the vendor’s instructions. (Ray- Biotech, Norcross, GA, USA; catalogue no. EIANES-1).