Ovarian rejuvenation for menopausal or perimenopausal women using autologous platelet rich plasma (A-PRP) injection into the ovaries, for women seeking in vitro fertilisation (IVF) treatment, aiming to improve IVF outcomes.

Autologous platelet-rich plasma (A-PRP) will be injected into the ovaries of perimenopausal and menopausal women. The PRP preparation kit is Alocuro PRO PRP 30mL device approved by the Therapeutics Goods Agency (TGA) listed on the Australian Register of Therapeutic Goods (ARTG) as a class II medical device, manufacturer - GoodmorningBio Co Ltd, Republic of Korea. The kit contains:*Venepuncture equipment; Butterfly needle / White top syringe / Yellow top syringe x 4 (each containing 1mL of anti-coagulant). *PRP Preparation equipment; 30mL PRO PRP device (centrifuging tube) / S-Monovette-needle. Trained phlebotomist's will draw 30mLs of blood as per the manufacturer's instructions. A scientist trained in the preparation of A-PRP will prepare the blood sample as per the manufacturer's instructions, 30mLs of blood will harvest around 4-5mLs of A-PRP. A FRANZCOG trained fertility specialist will inject the A-PRP into both ovaries via transvaginal ultrasound guidance or laparoscopy, using an oocyte pick-up (OPU) needle. If one ovary cannot be safely accessed, only one will be injected, around 2-2.5mLs of A-PRP will be injected per ovary. The injection will occur under either local anaesthetic (LA) or general anaesthetic (GA), either within a cycle of mild ovarian stimulation IVF at the time of the transvaginal oocyte aspiration (TVOA), or outside of a cycle of IVF in the follicular phase of menstruation, or for patients with amenorrhoea at any point. The intraovarian injection (IOI) of A-PRP may be repeated on subsequent IVF cycles. The blood collection and A-PRP preparation will take place in an RTAC / RTC / NATA accredited fertility clinic – Fertility North. The intraovarian injection will take place in a treatment room at Fertility North (if performed under LA) or Joondalup Health Campus hospital theatres (if performed under GA).