RecruitingNot ApplicableNCT03384108

In Vivo Assessment of Glutamine Utilization by Bone Marrow Plasma Cells

In Vivo Assessment of Glutamine Utilization by Bone Marrow Plasma Cells in Healthy Subjects Using Stable Isotope Resolved Metabolomics: A Pilot Study


Sponsor

Mayo Clinic

Enrollment

20 participants

Start Date

Sep 1, 2017

Study Type

INTERVENTIONAL

Conditions

Summary

This pilot study will first establish the feasibility of an in vivo methodology of assessing the utilization of glutamine into the TCA cycle of normal bone marrow plasma cells from healthy subjects while comparing it to an ex vivo approach


Eligibility

Min Age: 18 YearsMax Age: 60 Years

Inclusion Criteria2

  • Ages 18-60
  • Able to provide written consent

Exclusion Criteria7

  • Renal Failure
  • Pregnancy
  • Active steroid use
  • Active liver Disease
  • Anemia (Hemoglobin < 12.5 g/dL in men and < 11.5 g/dL in women)
  • H/O alcohol use (average > 2 drinks per day)
  • BMI > 30

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Interventions

OTHER5-13C-Glutamine

Intravenous infusion of 5-13C-Glutamine

OTHERBone marrow aspiration

Bone marrow aspiration of bone marrow aspirate


Locations(1)

Mayo Clinic

Rochester, Minnesota, United States

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NCT03384108


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