Mucosal IgE to Improve Diagnosis of Food Allergy and Food Hypersensitivity
Improving the Diagnosis of Food Allergy and Food Intolerance by Determining Mucosal IgE and Inflammatory Markers and Validating With Intestinal in Vitro Organoids
University of Erlangen-Nürnberg Medical School
115 participants
Feb 1, 2022
INTERVENTIONAL
Summary
Aim of the study is to improve the diagnosis of food allergy and hypersensitivity. Intestinal homogenates will be used to determine total IgE, specific IgE, tryptase, histamine and inflammation parameters (IFNgamma, TNFalpha). These data will be correlated with serum values and disease status. In addition, organoids from duodenal tissue will be isolated and cultured in vitro and stimulated with the major food allergens. The gene and protein expression will be checked to identify relevant biomarkers.
Eligibility
Inclusion Criteria3
- informed consent
- patients with suspected food allergy or hypersensitivity
- healthy controls with indications for endoscopic diagnostics, e.g. tumour history within the family, exclusion of gastritis
Exclusion Criteria1
- pregnant person
Interested in this trial?
Get notified about updates and connect with the research team.
Interventions
1. Biopsies are homogenised in buffer and used for the determination of mucosal IgE and inflammatory parameters. 2. Biopsies are used to isolate organoids, stimulate them with blood cells and food antigens and to study gene and protein expression.
Locations(1)
View Full Details on ClinicalTrials.gov
For the most up-to-date information, visit the official listing.
NCT05259826