RecruitingNCT05400590

Comparison of the Healing Properties on Corneal Cells of Groth Factor-enriched Plasma and Autologous Serum From Aniridia Patients


Sponsor

Fondation Ophtalmologique Adolphe de Rothschild

Enrollment

24 participants

Start Date

May 15, 2023

Study Type

OBSERVATIONAL

Conditions

Summary

Keratopathy of patients with aniridia leads to epithelial scarring disorders and a progressive clouding of the cornea linked to this abnormal healing (fibrosis). Treatment with autologous serum is usually undertaken to promote epithelial healing. However, autologous serum does not prevent the formation of fibrosis, whereas growth factor-rich plasma appears to be associated with a reduction in the in vitro expression of fibrosis markers. This study seeks to compare the in vitro healing and anti-fibrotic properties of autologous serum and growth factor rich plasma from aniridia patients and healthy controls.


Eligibility

Min Age: 18 Years

Inclusion Criteria11

  • For patients with aniridia:
  • With aniridia
  • Patient weighing more than 43 kg
  • If the patient is being treated with autologous serum, a delay of 8 weeks after the last blood collection
  • If a blood donation has been made, a delay of 8 weeks must be respected after the last donation
  • For controls:
  • Non-aniridia (no clinical signs)
  • Weighing more than 43 kg
  • Matched to a case for sex and age +/-10 years
  • No known diabetes
  • If a blood donation has been made, a delay of 8 weeks must be respected after the latter

Exclusion Criteria4

  • Insufficient sample volume
  • No conjunctival print
  • HBV, HCV, HIV serology positive
  • Aniridia not genetically confirmed for cases For each patient excluded, a new patient will be included in order to maintain the number of patients required for data analysis.

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Interventions

PROCEDUREPeripheral venous blood sample

2.5% of body weight

PROCEDUREConjunctival impression

Collection of the most superficial conjunctival epithelial cells (desquamating) in a non- or minimally invasive, quick and almost painless way, for a biological analysis of ocular surface diseases. Application of a filter paper on the bulbar conjunctiva, under local anesthesia.


Locations(1)

Hôpital Fondation A. de Rothschild

Paris, France

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NCT05400590


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