Autologous Testicular Tissue Transplantation

Inclusion Criteria12

• The eligible patients opted as a prepubertal boy to enroll in the fertility preservation program and on the moment of cancer diagnosis or hematological disorder, their parents have agreed to cryopreserve testicular tissue for later autologous transplantation.
• At least 18 years old
• Desire to become a parent at the moment of intake
• Stable relationship with a female partner and minimal one year cohabiting
• Age of female partner \< 43 year
• Azoospermia on 2 semen analyses
• Normal standardised preliminary and preoperative bloodsampling results
• Complete remission of the oncological or hematological disease
• Approval of the treating oncologist or other specialist in case of non-oncological disease as reason for the testicular tissue preservation as a child
• Risk for presence of malignant cells in testicular tissue is negligible (according to multidisciplinary assessment)
• Presence of SSCs (positive MAGE staining) in one or two of the thawed fragments (If absence of SSCs in two of the thawed fragments, the case will be discussed multidisciplinary)
• Written informed consent for the transplantation of cryopreserved testicular tissue and follow-up after the procedure and of children born eventually after this procedure