RecruitingPhase 1NCT05773001

Macrophage Regulation of Ozone-Induced Lung Inflammation


Sponsor

Robert Tighe, MD

Enrollment

100 participants

Start Date

May 18, 2023

Study Type

INTERVENTIONAL

Summary

The purpose of this research study to understand how prior respiratory infections affect the susceptibility to lung inflammation following environmental exposures.


Eligibility

Min Age: 18 YearsMax Age: 55 Years

Inclusion Criteria6

  • Individuals between 18-55 yrs. of age (No subject will be excluded from the study on the basis of gender or ethnicity)
  • Individuals with knowledge of prior SARS-CoV-2 infection history allowing them to be segregated into one of three cohorts
  • Cohort 1 - No history of SARS-CoV-2 infection (defined as no symptoms consisted with SARS-CoV-2 nor history of a positive SARS-CoV-2 test)
  • Cohort 2 - Documented mild SARS-CoV-2 infection (a positive test, either PCR- or antigen-based) but with mild to no symptoms and no evidence of a lower respiratory tract infection (including no hospitalization, and no oxygen use)
  • Cohort 3 - History of SARS-CoV-2 infection and symptoms/imaging consistent with a lower respiratory tract infection who have recovered, are >6 months out from their infection, and have normal lung function (spirometry with FVC, FEV1 and FEV1/FVC)
  • There will be no maximal period from SARS-CoV-2 infection for inclusion in the study, the minimal period will be >6 months out from infection

Exclusion Criteria10

  • Individuals with prior SARS-CoV-2 pneumonia who have ongoing respiratory symptoms, are still using supplemental oxygen, or have abnormal lung function
  • Individuals with prior SARS-CoV-2 infection that cannot provide documentation of a positive test
  • Current smokers of tobacco products including e-cigarettes or those with previous smoking history within the prior 5 years
  • Pregnant women and women who are presently lactating.
  • Subjects that have received antibiotic administration or an upper respiratory infection within the previous 4 weeks
  • College and graduate students or employees who are under direct supervision by any of the investigators in this protocol
  • Alcohol or illicit substance abuse
  • Chronic cardio/pulmonary respiratory disorders or other medical conditions as determined by the investigator
  • Increased airway hyperresponsiveness at baseline as measured by a positive methacholine challenge response (methacholine PC20 FEV1 < 4 mg/ml)
  • Subjects will be requested to refrain from antihistamines, nonsteroidal anti-inflammatory agents, antioxidants (e.g. beta-carotene, selenium, and lutein) and supplemental vitamins (e.g. C and E), for 1 week prior to, and during testing.

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Interventions

DRUGOzone

Subjects will perform alternating 15 minutes rest with 15 minutes treadmill walk exercise periods for 135 minutes in while breathing Ozone (O3).


Locations(1)

Duke University Medical Center

Durham, North Carolina, United States

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NCT05773001