RecruitingNot ApplicableNCT06031714

Fetal Cell Receptors Repertoire

"Study of CCR Receptor Overexpression in Fetal Microchimeric Cells: Proof of Concept Before a Potential Clinical Trial"

Sponsor

Assistance Publique - Hôpitaux de Paris

Enrollment

160 participants

Start Date

Feb 6, 2024

Study Type

INTERVENTIONAL

Conditions

Venous Ulcer Sickle Cell Ulcer Diabetic Ulcer Post-partum Women Mixed Ulcer

Summary

The purpose of this study is to describe the transcriptomic profile of foetal cells in post-partum and more specifically to determine which chemokine receptors are overexpressed in foetal cells in post-partum women with wounds To do so, the investigators will isolate foetal cells from the peripheral blood of healthy controls post partum women as well as from post partum women with skin ulcers and then perform RNA sequencing.

Eligibility

Inclusion Criteria13

Common criteria :
Adult women,
Post-partum: having been pregnant for any length of time,
Having signed a free and informed consent form,
Primiparous or multiparous,
Affiliated to a health insurance
Patients :
\- Patients with a venous, diabetic or sickle cell ulcer, or mixed ulcer
Control group patients :
Volunteers,
Age-matched,
Without skin ulcers.
There are no specific criteria for children.

Exclusion Criteria5

Minors (for patients)
Under court protection, curatorship, guardianship (for patients)
Immunocompromised patients for any reason whatsoever
Refusal of consent
Refusal of blood and/or saliva samples for themselves or a member of their family

Interested in this trial?

Get notified about updates and connect with the research team.

Interventions

OTHERSaliva sampling

HLA genotyping. The technique should allow to identify, for children's, a paternal HLA antigen not shared with the mothers.

OTHERBlood sampling

Maternal Blood samples will be incubated with the appropriate antibody, targeting the microchimeric fetal cells of each patient, as well as with a cell viability marker (DAPI). The samples were then be processed through the BD FACS Aria III to sort the fetal cells, The following steps - RNA extraction, quality control, retrotranscription, preparation of the library, sequencing and transcriptomic analysis - will be carried out according to the Smart-seq3 protocol. The data will be sent for in-depth analysis and confirmation of the results. Additional functional experiments may also be carried out.

OTHERInterviews

V2 and/or V3

OTHERClinical examination

V2 and/or V3