RecruitingPhase 1NCT06121570

Phase I Study of Autologous Tumor-Draining Lymph Node-Derived Lymphocytes as Neoadjuvant Therapy for HER2-Negative Breast Cancer


Sponsor

Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University

Enrollment

24 participants

Start Date

Nov 1, 2023

Study Type

INTERVENTIONAL

Conditions

Summary

RATIONALE: Patients with HER2-negative breast cancer not responding to initial neoadjuvant chemotherapy might have lower chances for a pathologic complete response (pCR) at definitive surgery, indicating worse prognosis. Adoptive cell therapy has demonstrated efficacy in advanced breast cancer, but whether the addition of adoptive cell therapy to neoadjuvant chemotherapy could increase the pCR rate remains unclear. Tumor-draining lymph node-derived lymphocytes (LNLs) that have abundant tumor-reactive T cells, but not exhausted T cells, are easy to produce. It is not yet known whether LNL treatment is safe and effective in patients with HER2-negative breast cancer not responding to neoadjuvant chemotherapy. PURPOSE: This phase I trial is mainly to investigate the safety of autologous LNL in patients with HER2-negative breast cancer not responding to neoadjuvant chemotherapy.


Eligibility

Sex: FEMALEMin Age: 18 YearsMax Age: 70 Years

Plain Language Summary

Simplified for easier understanding

This study tests a new approach to treating HER2-negative breast cancer before surgery (called neoadjuvant therapy). Immune cells are taken from nearby lymph nodes, grown in the lab, and infused back into the patient to boost the immune response against the tumor before the main surgical procedure. **You may be eligible if...** - You are a woman aged 18–70 with confirmed invasive HER2-negative breast cancer - Your primary tumor is at least 1 cm - You have already completed 2 cycles of standard chemotherapy (doxorubicin/epirubicin + cyclophosphamide) and your disease is stable - You have not had prior breast cancer treatment (other than the 2 chemotherapy cycles mentioned above) - You are in good overall health (ECOG 0 or 1) **You may NOT be eligible if...** - Your cancer is HER2-positive - You have already had sentinel lymph node biopsy or full underarm lymph node removal for this cancer - You have previously received radiotherapy, hormonal therapy, targeted therapy, or other immunotherapy for breast cancer - Your blood counts, liver, or kidney function are not adequate Talk to your doctor to see if this trial is right for you.

This summary was AI-generated to explain the trial in plain language. It is not medical advice. Always discuss eligibility with your doctor before enrolling in a clinical trial.

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Interventions

PROCEDURESentinel Lymph Node Biopsy (SLNB)

A sample of the participant's tumor-draining lymph nodes will be collected and sent to the biotherapy center for LNL isolation and expansion.

DRUGDoxorubicin

Doxorubicin will be administered at 60 mg/m\^2 IV on Day 1 of Cycles 1-2 of the neoadjuvant chemotherapy of the study.

DRUGEpirubicin

Epirubicin will be administered at 100 mg/m\^2 IV on Day 1 of Cycles 1-2 of the neoadjuvant chemotherapy of the study.

DRUGCyclophosphamide

Cyclophosphamide will be administered at 600 mg/m\^2 IV on Day 1 of Cycles 1-2 of the neoadjuvant chemotherapy of the study.

DRUGCyclophosphamide

Cyclophosphamide will be administered at 500 mg/m\^2 IV daily for three days. Cyclophosphamide will be initiated five days prior to the anticipated LNL transfer, and the precise timing will depend on the rate of in vitro LNL growth.

DRUGFludarabine

Fludarabine will be administered at 30 mg/m\^2 IV daily for three days. Fludarabine will be initiated five days prior to the anticipated LNL transfer, and the precise timing will depend on the rate of in vitro LNL growth.

BIOLOGICALTumor-draining lymph node-derived lymphocyte (LNL)

Participants receive single infusion of LNL at the recommended phase 2 dose (RP2D).

BIOLOGICALInterleukin-2

Eight to twelve hours after completing the LNL infusion, all participants will receive intermediate-dose decrescendo IL-2 IV.

DRUGNab-paclitaxel

Nab-paclitaxel will be administered at 260 mg/m\^2 IV on Day 1 of Cycles 3-6 of the neoadjuvant chemotherapy of the study.


Locations(1)

Sun Yat-sen Memorial Hospital, Sun Yat-sen University

Guangzhou, Guangdong, China

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NCT06121570


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