RecruitingPhase 4NCT06146088

Lot-to-lot Consistency Study of Three Commercial Batches of Enterovirus 71 Vaccine

To Evaluate the Lot-to-lot Consistency of Immunogenicity, Safety, and Immune Persistence of Three Consecutive Commercial Manufacturing Batches of Inactivated Enterovirus 71 Vaccine (Human Diploid Cell) in Children Aged 6-35 Months in a Randomized, Blinded Study


Sponsor

Institute of Medical Biology, Chinese Academy of Medical Sciences

Enrollment

1,500 participants

Start Date

Nov 25, 2023

Study Type

INTERVENTIONAL

Conditions

Summary

This study is a randomized, blinded study to evaluate the lot-to-lot consistency of immunogenicity, safety, and immune persistence of three consecutive manufacturing lots of EV71 vaccine, in 1500 children aged 6-35 months. The primary immunogenicity endpoint is the anti-EV71 neutralizing antibody geometric mean titer (GMT) 30 days after the final dose. The secondary immunogenicity endpoints are the geometric mean fold increases and seroconversion rates of anti-EV71 neutralizing antibodies 30 days after the final dose. The immune persistence endpoints are the seropositive rates as well as GMT of anti-EV71 neutralizing antibodies 12 and 24 months after the final dose. The safety endpoints are the number of adverse events/reactions within 30 minutes after each dose, the number of solicited adverse events/reactions within 7 days after each dose, the number of unsolicited adverse events/reactions within 30 days after each dose, and the number of serious adverse events (SAE) from the first dose to 6 months post the final dose.


Eligibility

Min Age: 6 MonthsMax Age: 35 Months

Inclusion Criteria4

  • Age Requirement: Children aged 6 to 35 months at the time of enrollment.
  • Provision of legal Identification: Volunteers and their legal guardians or appointed representatives must provide valid legal identification documents.
  • Informed Consent: Legal guardians or appointed representatives of volunteers must have the capacity to understand the informed consent document and the research process, voluntarily participate, sign the informed consent form, and be able to comply with the study's requirements and complete relevant visits on time.
  • Temperature Requirement: Axillary body temperature prior to vaccination is less than 37.3°C.

Exclusion Criteria16

  • Allergic History: Subjects have a previous history of allergies to any component of the vaccine (e.g., aluminum hydroxide, glycine), or previous or suspected allergy to any vaccine or other serious adverse reaction (e.g., anaphylactic shock, laryngeal edema, anaphylactic purpura, thrombocytopenic purpura, local anaphylactic necrosis reaction, dyspnea, angioedema, systemic rash and/or urticaria, etc.).
  • Vaccination History: Subjects received any inactivated vaccine or subunit vaccine within 7 days prior to the first dose of the investigational vaccine or any live attenuated vaccine within 14 days prior to the first dose of the investigational vaccine.
  • Neurological and Mental Health: Subjects with a history or family history of convulsion, epilepsy, encephalopathy, and psychosis.
  • Health Conditions: Subjects with known severe congenital malformation or developmental disorder (e.g., Down syndrome, sickle cell anemia, congenital neurological disorders).
  • Acute Illness: Subjects have experienced acute illness (e.g., fever) or acute onset of chronic illness within 3 days before the first dose of investigational vaccine, or use of antipyretic, analgesic, or antiallergic drugs within 3 days before the first dose of the investigational vaccine.
  • Coagulation Abnormalities: Subjects with genetic bleeding tendency, coagulopathy, or a history of bleeding disorders.
  • Organ Removal History: Subjects have a history of surgical removal of the spleen or other vital immune organs for any reason.
  • Blood Conditions: Subjects with blood loss (≥400mL) or receipt of blood transfusion, immune globulin (other than hepatitis B immune globulin), or blood products within 3 months prior to the first dose of the investigational vaccine.
  • Drug Administration: Subjects use any investigational or unregistered product (drug, biological product, or device) within 3 months prior to the first dose of the investigational vaccine, or planned use during the study, except the investigational vaccine.
  • Immune Therapy: Subjects have a treatment with immunosuppressive agents within 6 months before the first dose of the investigational vaccine, such as long-term treatment with systemic glucocorticoids (e.g., prednisone or a similar agent for more than 2 consecutive weeks within 6 months), but topical use (e.g., ointments, eye drops, inhalers, or nasal sprays) would be allowed.
  • Previous history of receipt of other EV71 vaccines.
  • Previous history of hand-foot-mouth disease.
  • Immune-related Disease: Subjects have been diagnosed with an infectious disease that may interfere with the conduct or completion of the study (e.g., tuberculosis, viral hepatitis, human immunodeficiency virus infection, etc.).
  • Investigators' Discretion: The final exclusion criterion is the investigator's discretion to determine whether a subject is suitable for participation in the study.
  • Severe Adverse Reactions: Subjects experienced serious adverse reactions after receiving the previous vaccine dose.
  • Other Exclusion Reasons as Determined by the Investigator: The investigator determines other reasons for exclusion.

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Interventions

BIOLOGICALEV71 Vaccine

Inactivated enterovirus 71 vaccine (human diploid cell) of 3.0EU/0.5mL on Day 0,30


Locations(4)

Chencang Center for Disease Prevention and Control

Baoji, Shaan XI, China

Ningqiang Center for Disease Prevention and Control

Hanzhong, Shaan XI, China

Fuping Center for Disease Prevention and Control

Weinan, Shaan XI, China

Jingyang Center for Disease Prevention and Control

Xianyang, Shaan XI, China

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NCT06146088


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