Insights Into Tick-Borne Diseases: a Study From an Endemic Area of Northern Italy
Insights Into Tick-Borne Diseases: a Prospective Study From an Endemic Area of Northern Italy
IRCCS Sacro Cuore Don Calabria di Negrar
384 participants
Mar 25, 2024
INTERVENTIONAL
Conditions
Summary
This is a monocentric, experimental, non-pharmacological and non-device no profit study. The study is experimental because all the visits and blood withdrawals foreseen by the protocol do not fall within the normal clinical practice.
Eligibility
Inclusion Criteria2
- all individuals aged > 8 years presenting for tick removal.
- Individuals providing signed informed consent.
Exclusion Criteria1
- tick not available for analysis
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Interventions
Ticks will be classified based on morphological characteristics by microscopy observation. For TBP detection by molecular test, Nucleic acid extraction will be performed on ticks lysate or blood using kits MagMAX TM Viral/Pathogen Nucleic Acid Isolation Kit (Thermo Fisher Scientific) on MagEX STARlet platform from Hamilton. Species identification by molecular method will be performed by Sanger sequencing for Rickettsiae and Babesia, and Multi-Locus Sequence Typing will be used to characterize Borrelia. Serum samples collected throughout the study will be tested to assess any seroconversions during the follow-up period. Samples will be tested for the presence of specific IgG and IgM for the following TBPs: TBEV, R. typhi. R. conorii, Borrelia spp, Anaplasma, Ehrlichia and Babesia.
Locations(1)
View Full Details on ClinicalTrials.gov
For the most up-to-date information, visit the official listing.
NCT06374615