RecruitingNot ApplicableNCT06533215

Antimicrobial Efficacy of Nano-Based ICM on E.F

Antimicrobial Efficacy of Nano-Based Intracanal Medications on Enterococcus Faecalis

Sponsor

Tanta University

Enrollment

30 participants

Start Date

Jan 2, 2024

Study Type

INTERVENTIONAL

Conditions

Endodontic Disease Endodontically Treated Teeth Enterococcus Faecalis Infection

Summary

This in-vivo study aims to evaluate the antimicrobial effect of nano based intracanal medications (Triple antibiotic-loaded chitosan nanoparticles and chlorohexidine loaded by silver nanoparticles) on Enterococcus faecalis count reduction in secondary endodontic infection cases.

Eligibility

Min Age: 19 YearsMax Age: 60 Years

Inclusion Criteria6

Single rooted, single canal premolar teeth.
Teeth indicated for non-surgical root canal retreatment.
Teeth have signs and/or symptoms of post-treatment disease manifested by recurrent acute and/or chronic periapical abscess.
Teeth have clinical signs and symptoms of endodontic failure as sensitivity to percussion, pain, swelling or fistula.
Teeth with radiographic features of endodontic failure as persistent periapical lesion or widening of periodontal ligament.
Symptomatic or asymptomatic teeth with short root canal filling indicated for prosthetic restoration

Exclusion Criteria8

Patients with any systemic diseases.
Pregnant or lactating patients.
Immunocompromised patients.
Non-restorable tooth.
Calcified root canal.
Teeth with root fracture.
Teeth with periodontal pocket deeper than 4mm.
Teeth have procedural error like ledge, broken instrument, or perforation

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Interventions

OTHERantimicrobial efficacy of nano based intracanal medications on Enterococcus faecalis count reduction in secondary endodontic infection cases.

The paper points of three samples (S1, S2, and S3) will be placed each in a sterile falcon tube containing 1ml of brain heart infusion and labeled according to the type of intracanal medication used. Samples will be homogenized by the vortex and and one micron of each sample will be cultured on Bile Esculin Agar for E. faecalis. The plate will be labeled as the tube of the same sample and incubated at 37οC for 24-48 h and then after incubation, the total numbers of E.faecalis will be counted and calculated to get the colony forming units (CFU/ml).