RecruitingNCT06694363

New Biomarker-based Strategy to Screen and Monitor for Activated Phosphoinositide 3-kinase δ Syndrome


Sponsor

Assistance Publique - Hôpitaux de Paris

Enrollment

14 participants

Start Date

Jun 26, 2025

Study Type

OBSERVATIONAL

Conditions

Summary

The study would like to compare patient samples at different time points using state-of the art-phenotyping tools. Collection of blood samples of APDS patients undergoing PI3K inhibitor treatment will be collected when feasible according to the standard of care planning (a blood test is supposed to be performed for these patients at M0-M3-M6-M12 then each 6 months for a total period of 2 years from the beginning of the PI3K inhibitor treatment). The whole blood will be processed in order to isolate the peripheral blood mononuclear cells (PBMC) and the plasma. Serum, RNA and DNA extraction will be performed on a separate sample.


Eligibility

Min Age: 12 Years

Inclusion Criteria12

  • Group 1:
  • Patients with genetic diagnosis of APDS type 1 or type 2 and planned to be treated by PI3Kδ selective inhibitor leniolisib
  • Primary immunodeficient patients with new disease-causing variants in the PIK3CD gene or PIK3R1 gene
  • Minimum age 12 years old
  • Patients or holders of parental authority do not oppose participation in this research.
  • Patients affiliated to a Health Insurance scheme or beneficiaries
  • Group 2 :
  • Patients with genetic diagnosis of APDS type 1 or type 2 already treated by PI3Kδ selective inhibitor leniolisib in the last 2 years
  • Patients whose pre-treatment samples are available/analyzable
  • Minimum age 12 years old
  • Patients or holders of parental authority do not oppose participation in this research.
  • patients affiliated to a Health Insurance scheme or beneficiaries

Exclusion Criteria2

  • Bone marrow transplantation
  • Refusal to participate to the study.

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Interventions

BIOLOGICALBlood samples

A maximum of 27 ml of blood collected at each visit for metabolic markers analysis

BIOLOGICALUrine samples

One urine sample collected at each visit for enteric virus infection research

BIOLOGICALStool samples

One stool sample collected at each visit for enteric virus infection research


Locations(7)

Hôpital Haut Levêque - BORDEAUX

Bordeaux, France

Hôpital Pellerin Enfants - BORDEAUX

Bordeaux, France

Hôpital Jeanne de Flandres - LILLE

Lille, France

Hôpital La Timone adulte - MARSEILLE

Marseille, France

Hôpital Necker Enfants Malades - PARIS

Paris, France

CHU IUCT Oncopole - TOULOUSE

Toulouse, France

Hôpital des enfants - TOULOUSE

Toulouse, France

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NCT06694363