Transcriptomics as an Aid in the Histological Diagnosis of Acute Rejection After Liver Transplantation
IRCCS Azienda Ospedaliero-Universitaria di Bologna
40 participants
Oct 17, 2024
INTERVENTIONAL
Conditions
Summary
The primary objective of this exploratory study is to identify genes associated with acute rejection in liver transplant patients. To this end, a panel of acute rejection indicator genes (both humoral and cell-mediated) will be selected from results published in the literature and applied to biological material derived from biopsies. The secodnary objective is to identify genes associated with the diagnosis of humoral acute rejection and those associated with the diagnosis of cell-mediated acute rejection.
Eligibility
Inclusion Criteria3
- Patients with a diagnosis of post-OLT rejection prior to the visit at which they will be enrolled, with tissue available for RT-PCR investigations
- Age ≥ 18 years
- Informed consent
Exclusion Criteria1
- Age <18 years
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Interventions
RNA will be analysed using a Real-Time PCR expression card (TaqMan Real-Time PCR assay technology - Thermo Fisher Scientific). The analysis will be performed using a Real-Time Light Cycler 480 (Roche). The expression card will be customised for the study, including the genes cxcl9/10, cav-1, rhoj, cdh5, cd96, tnfsf8, il-21r, vcam, klrk1
Locations(1)
View Full Details on ClinicalTrials.gov
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NCT06734013