RecruitingNCT06919393

"Triple Negative" Adult B-cell Acute Lymphoblastic Leukemia - TRINEG-ALL

"Triple Negative" Adult B-cell Acute Lymphoblastic Leukemia: Molecular Characterization, Identification and New Stratification


Sponsor

Istituto Romagnolo per lo Studio dei Tumori Dino Amadori IRST S.r.l. IRCCS

Enrollment

60 participants

Start Date

Oct 23, 2019

Study Type

OBSERVATIONAL

Conditions

Summary

It is a multicenter, non-interventional, non pharmacological, translational, prospective study. Any decision about drug administration is made by the physician based on his clinical judgment in the context of clinical practice, independently from the decision to include the patient in the study.


Eligibility

Min Age: 18 Years

Inclusion Criteria6

  • Participant is willing and able to give informed consent for participation in the study;
  • Patients with new diagnosis and/or relapse/s of primary or secondary B-ALL;
  • Negative for BCR-ABL1 t(9;22); TCF3-PBX1 t(1;19); MLL-AF4 t(4;11) rearrangements;
  • Participant is willing and able to give informed consent for participation in the study;
  • Male or Female, aged \>18 years;
  • Availability of clinical data.

Exclusion Criteria3

  • Age \< 18 years;
  • B-ALL positive for BCR-ABL1 t(9;22); TCF3-PBX1 t(1;19); MLL-AF4 t(4;11) rearrangements.
  • Low blast percentage (\<70%) samples could be excluded for molecular evaluations, not for cytofluorimetric analyses;

Interventions

OTHER"Triple negative" adul B-cell ALL

Samples will be studied with conventional techniques to classify and define properly the disease: morphology, immunophenotype, immunohistochemistry (IHC), conventional Cytogenetic Fluorescence in Situ Hybridization (FISH) will be used when appropriate. ● Isolation of Mononuclear cells The following research methodologies will be applied: * Next Generation Sequencing * Flow cytometry analysis of 3C-up B-ALL top three markers * Gene expression profile analysis * Copy number Alterations analysis * In vitro studies


Locations(3)

Irst Irccs

Meldola, FC, Italy

Ospedale S. Maria delle Croci RAVENNA

Ravenna, RA, Italy

Ospedale Infermi

Rimini, RN, Italy

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NCT06919393


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