RecruitingNot ApplicableNCT07050537

A Multicenter Prospective Randomized Controlled Clinical Trial Comparing Constant (5%) Versus Sequential (5%-2%) Oxygen Concentration Embryo Culture Protocols in Assisted Reproductive Technology

A Multicenter Prospective Randomized Controlled Clinical Trial Comparing Constant (5%) Versus Sequential (5%-2%) Oxygen Concentration Embryo Culture Protocols in Assisted Reproductive Technology: Evaluating Term Live Birth and Offspring Health Outcomes


Sponsor

The First Affiliated Hospital with Nanjing Medical University

Enrollment

980 participants

Start Date

Mar 1, 2025

Study Type

INTERVENTIONAL

Conditions

Summary

To evaluate whether there were significant differences in the effects of constant (5%) versus sequential (5%-2%) oxygen concentration protocols in embryo culture on term live birth rates. This study will be conducted in five centers including the Clinical Center of Reproductive Medicine, First Affiliated Hospital of Nanjing Medical Universit. 980 women who wish to undergo blastocyst transplantation will be included in the study. The study data will be analyzed statistically.


Eligibility

Sex: FEMALEMin Age: 20 YearsMax Age: 40 Years

Inclusion Criteria3

  • Female: age ≥20 and ≤40 years, male: age ≥20 and ≤50 years;
  • Proposed 1st or 2nd cycle of IVF or ICSI fertilization;
  • ≥ 4 transferable embryos at oocyte stage.

Exclusion Criteria7

  • Diagnosis of abnormal uterine cavity morphology (confirmed by 3D ultrasound or hysteroscopy), including uterine malformations (mediastinal uterus, unicornuate uterus, bicornuate uterus), submucosal uterine fibroids, or uterine adhesions;
  • Patients who are proposed to undergo IVM;
  • Patients who are proposed to undergo PGD/ PGS;
  • Patients with untreated severe hydrosalpinx (confirmed by ultrasound or HSG);
  • Patients with a history of recurrent miscarriage (2 or more previous pregnancy losses, excluding biochemical pregnancies);
  • Patients who plan to freeze whole embryos and are unable to complete a single embryo transfer within six months;
  • Patients with contraindications to assisted reproductive technology and pregnancy, or suffering from diseases that have a definite effect on pregnancy: including uncontrolled hypertension, heart disease with definite symptoms, uncontrolled diabetes, undefined diagnosis of liver or kidney disease or liver or kidney insufficiency, severe anemia, history of previous venous thrombosis, pulmonary embolism or cerebrovascular events, history of malignant tumors, suspected malignant tumors, and undiagnosed abnormal uterine bleeding.

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Interventions

PROCEDUREConstant oxygen concentration protocols in embryo culture

Participants were randomly assigned to constant (5%) oxygen concentration group and received different blastocyst culture protocols.

PROCEDURESequential oxygen concentration protocols in embryo culture

Participants were randomly assigned to sequential (5%-2%) oxygen concentration group and received different blastocyst culture protocols.


Locations(5)

The Third Affiliated Hospital of Zhengzhou University

Zhengzhou, Henan, China

Changzhou Maternal And Child Health Care Hospital

Changzhou, Jiangsu, China

Nanjing Women and Children's Healthcare Hospital

Nanjing, Jiangsu, China

Reproductive Medicine of First Affiliated Hospital with Nanjing Medical University

Nanjing, Jiangsu, China

Suzhou Municipal Hospital

Suzhou, Jiangsu, China

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NCT07050537


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