Developing Remote Human Milk Collection as a Novel Technique for Transcriptome Sequencing
University of Wisconsin, Madison
50 participants
Nov 14, 2025
INTERVENTIONAL
Conditions
Summary
This study will develop a protocol for remote human milk collection for use in gene expression research through application of ribonucleic acid (RNA) preservation reagents. The investigators hypothesize that addition of preservation reagents will preserve RNA in human milk, facilitating downstream analyses that require intact RNA. 50 lactating participants will be recruited and requested to provide reagent-mixed samples to be sent to the university through shipping services where they will be evaluated for RNA quantity, quality and profile.
Eligibility
Inclusion Criteria2
- English speaking and reading
- Lactating for greater than or equal to 4 weeks
Exclusion Criteria2
- not meeting personal lactation goals
- resides outside of Wisconsin
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Interventions
Two products will be evaluated for their ability to preserve RNA in human milk samples. * RNAlater (ThermoFisher©) (ratio variable) * NucleoProtect RNA (Takara©) (ratio variable)\* * Product volume mixed with human milk samples will vary by product and may be titrated as part of protocol development.
Locations(1)
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NCT07118852