RecruitingNCT07171151

Detection of Disease Marker Factors in Cyst Fluid

Measurement of MMP-8, MMP-9, RANKL, MIP-1alpha in Biopsy Samples From Odontogenic Cyst Patients

Sponsor

Harran University

Enrollment

25 participants

Start Date

Aug 25, 2025

Study Type

OBSERVATIONAL

Conditions

Biomarkers

Summary

The purpose of this observational study is to examine the cyst fluid obtained after a puncture procedure performed for cyst diagnosis in participants with cystic lesions on clinical and radiological examination.The main question it aims to answer is: \- What is the exact mechanism of formation of odontogenic cysts? Biomarkers in the cyst fluid obtained after a puncture procedure performed as part of the diagnosis and treatment process of odontogenic cysts will be examined. The aim of this study is to gain insight into cyst pathology by examining the levels of biomarkers in odontogenic cyst fluid.

Eligibility

Min Age: 12 YearsMax Age: 80 Years

Inclusion Criteria3

Individuals diagnosed with cysts
Individuals who are not pregnant or breastfeeding
Individuals who agree to sign the informed consent form

Exclusion Criteria3

Systemically ill individuals who are contraindicated for surgery.
Individuals who do not wish to sign the informed consent form.
Individuals who are pregnant, breastfeeding, or using oral contraceptives will be excluded.

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Interventions

OTHERcyst fluid examination

Participants will not undergo any procedures outside of the routine for the study. The cyst fluid obtained during the puncture will be collected in a tube and centrifuged at 3000 rpm for 20 minutes at 4 degrees Celsius. The Eppendorf tubes will be stored at -80 °C until the clear liquid remaining above is taken and examined, without removing the particles that have settled at the bottom of the tube. The biochemical parameters of the cyst fluid obtained from the study participants will be analyzed using the ELISA method. MMP-8, MMP-9, RANKL, and MIP1alpha will be analyzed using commercially available ELISA kits, following the manufacturer's instructions.