RecruitingNot ApplicableNCT07183488

Metabolic Effects of Short-Chain Fatty Acids in Healthy Individuals


Sponsor

University of Copenhagen

Enrollment

10 participants

Start Date

Sep 10, 2025

Study Type

INTERVENTIONAL

Conditions

Summary

The primary objective of this study is to investigate the acute physiological and metabolic effects of ingesting the short-chain fatty acids (SCFA)s propionate (three carbon atoms long; C3:0) and butyrate (C4:0) in healthy men and women. This aim will be addressed through a block-randomized cross-over study including two visits with acute intake of propionate and butyrate, respectively. The hypothesis is that plasma ketone body levels will increase during the first three hours after intake of butyrate, but not propionate, in healthy individuals in the overnight fasted state.


Eligibility

Min Age: 18 YearsMax Age: 40 Years

Inclusion Criteria3

  • Male or female.
  • Age between 18-40 years old
  • BMI between 18.5-29

Exclusion Criteria10

  • Diabetes
  • Kidney- or liver disease
  • Pregnant, lactating or planning to become pregnant within the study period
  • Supplementation with SCFAs
  • Supplementation with B12 vitamin
  • Special dietary habits (e.g. vegan/ketogenic diet)
  • Ongoing cancer treatment
  • Metabolic or absorptive disorders, gastric bypass operation, or use of medication affecting metabolism or food absorption
  • Crohn's disease, ulcerative colitis or short bowel syndrome
  • Inability, physically or mentally, to comply with the procedure required by the study protocol as evaluated by the primary investigator, study manager or clinical responsible

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Interventions

OTHERPropionate followed by Butyrate

The participants will consume Short-chain fatty acid propionate (SCFA-PRO) followed by Short-Chain Fatty Acid Butyrate (SCFA-BUT).

OTHERButyrate followed by Propionate

• The participants will consume Short-chain fatty acid Butyrate (SCFA-BUT) followed by Short-Chain Fatty Acid Propionate (SCFA-PRO).

OTHERPropionate followed by Butyrate

• The participants will consume Short-Chain Fatty Acid propionate (SCFA-PRO) followed by Short-Chain Fatty Acid Butyrate (SCFA-BUT).

OTHERButyrate followed by Propionate

The participants will consume Short-Chain Fatty Acid Butyrate (SCFA-BUT) followed by Short-Chain Fatty Acid Propionate (SCFA-PRO).


Locations(1)

University of Copenhagen

Copenhagen, Copenhagen N, Denmark

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NCT07183488


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