RecruitingNCT07491328

Liquid Biopsy in Paraneoplastic Neurological Syndromes

Liquid Biopsy in Paraneoplastic Neurological Syndromes With High- and Intermediate-Risk Antibodies


Sponsor

Heinrich-Heine University, Duesseldorf

Enrollment

60 participants

Start Date

May 20, 2023

Study Type

OBSERVATIONAL

Conditions

Summary

The main objective of the study is to assess whether circulating tumor cells (CTCs) can be detected in the peripheral blood of patients with paraneoplastic neurological syndromes (PNS) by diagnostic leukapheresis (DLA) combined with the CELLSEARCH® System during clinical routine workup. If CTCs can be identified, a detailed molecular genetic analysis will be performed to characterize mutations in oncogenes and tumor suppressor genes. In addition, the study aims to analyze cell-free DNA (cfDNA) in serum of patients. Individuals with PNS and high- or intermediate-risk or GAD65-antibodies with a detectable tumor (PNS+T), PNS patients with high- or intermediate-risk or GAD65-antibodies without detectable tumor (PNS w/o T), and matched tumor patients without PNS (T w/o PNS) will be included in the study.


Eligibility

Min Age: 18 Years

Inclusion Criteria2

  • Patients with paraneoplastic neurological syndromes (PNS) and high- or intermediate-risk or GAD65-antibodies or
  • Patients with peripheral tumors without PNS

Exclusion Criteria3

  • Age below 18 years at the time of inclusion.
  • Any mental condition impairing the capacity to provide informed consent.
  • Presence of contraindications to DLA.

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Interventions

DIAGNOSTIC_TESTCTC & ctDNA analysis

Analysis of circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) in the peripheral blood


Locations(1)

Department of Neurology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf

Düsseldorf, Germany

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NCT07491328