RecruitingPhase 1Phase 2NCT07508605

Efficacy and Safety of CD19/CD20 CAR/TRuC-T in Relapsed/Refractory B-Cell Lymphoma


Sponsor

Shenzhen University General Hospital

Enrollment

20 participants

Start Date

Jan 1, 2025

Study Type

INTERVENTIONAL

Conditions

Summary

1. Study Title: A Study on the Efficacy and safety of CD19/CD20 CAR/TRuC-T in Relapsed/Refractory B-Cell Lymphoma 2. Study Objectives: Primary Objective: To evaluate the safety of CD19/CD20 CAR/TRuC-T cell therapy in patients with relapsed/refractory B-cell lymphoma. Secondary Objective: To evaluate the efficacy of CD19/CD20 CAR/TRuC-T cell therapy in patients with relapsed/refractory B-cell lymphoma. Exploratory Objective: To assess in vivo expansion and persistence of infused CD19/CD20 CAR/TRuC-T cells. 3. Participant Intervention: Participants will receive lymphodepleting chemotherapy (FC regimen: Fludarabine + Cyclophosphamide) on Days -5, -4, and -3 relative to the planned CD19/CD20 CAR/TRuC-T cell infusion. The CAR/TRuC-T cell infusion will be administered 72 hours after the completion of the FC chemotherapy.


Eligibility

Min Age: 18 YearsMax Age: 75 Years

Inclusion Criteria12

  • Subjects must meet all of the following criteria to be enrolled:
  • Aged 18 to 75 years, regardless of sex;
  • Histologically confirmed relapsed/refractory B-cell lymphoma according to the 2020 World Health Organization (WHO) classification;
  • ECOG performance status of 0-2;
  • Expected survival of at least 3 months;
  • CD20 expression on tumor cells confirmed by flow cytometry and/or immunohistochemistry;
  • Patients who are resistant/refractory to CD19 CAR-T cell therapy or have low CD19 expression;
  • No severe cardiac, pulmonary, hepatic, or renal disease;
  • Able to understand and willing to sign the informed consent form for this study;
  • No contraindications to peripheral blood mononuclear cell collection/apheresis;
  • At least one measurable and evaluable lesion according to RECIST 1.1;
  • Must have previously received standard first-line and second-line therapy;

Exclusion Criteria21

  • Subjects meeting any of the following criteria will be excluded:
  • History of allergy to any component of the cell product;
  • Abnormal complete blood count meeting any of the following: WBC ≤1 × 10⁹/L, ANC ≤0.5 × 10⁹/L, ALC ≤0.5 × 10⁹/L, or PLT ≤25 × 10⁹/L;
  • Laboratory abnormalities including, but not limited to, any of the following: total serum bilirubin ≥1.5 mg/dL; ALT or AST >2.5 times the upper limit of normal; serum creatinine ≥2.0 mg/dL;
  • New York Heart Association (NYHA) Class III or IV heart failure, or left ventricular ejection fraction (LVEF) <50% on echocardiography;
  • Abnormal pulmonary function, with oxygen saturation <92% on room air;
  • History of myocardial infarction, cardiac angioplasty or stenting, unstable angina, or other clinically significant severe cardiac disease within 12 months prior to enrollment;
  • Grade 3 hypertension with poor blood pressure control despite medication;
  • History of craniocerebral trauma, disturbance of consciousness, epilepsy, severe cerebral ischemia, or cerebral hemorrhagic disease;
  • Presence of autoimmune disease, immunodeficiency, or other conditions requiring immunosuppressive therapy;
  • Presence of uncontrolled active infection;
  • Prior treatment with any CAR-T cell product or other genetically modified T-cell therapy;
  • Receipt of a live vaccine within 4 weeks prior to enrollment;
  • Positive for HIV, HBV, HCV, or TPPA/RPR, or HBV carrier status;
  • History of alcohol abuse, drug abuse, or psychiatric illness;
  • Participation in any other clinical study within 3 months prior to enrollment in this study;
  • Female subjects meeting any of the following conditions:
  • currently pregnant or breastfeeding;
  • planning to become pregnant during the study period; or
  • of childbearing potential and unwilling or unable to use effective contraception;
  • Any other condition that, in the investigator's judgment, makes the subject unsuitable for participation in this study.

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Interventions

COMBINATION_PRODUCTCAR/TRuC-T

Participants will receive lymphodepleting chemotherapy (FC regimen: Fludarabine + Cyclophosphamide) on Days -5, -4, and -3 relative to the planned CD19/CD20 CAR/TRuC-T cell infusion. The CAR/TRuC-T cell infusion will be administered 72 hours after the completion of the FC chemotherapy.


Locations(1)

Shenzhen University General Hospital

Shenzhen, Guangdong, China

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NCT07508605


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