Monitoring Changes in Blood Circulating Metabolites Using Minimally Invasive Techniques
Örebro University, Sweden
35 participants
Jan 21, 2026
INTERVENTIONAL
Conditions
Summary
This study investigates short-term intra- and inter-individual variability in human blood metabolism under real-life conditions. Using minimally invasive finger-prick blood sampling, dietary records, and wearable devices, we will longitudinally monitor blood-circulating metabolites and glucose levels in healthy individuals over a four-week period without dietary or lifestyle intervention. The study focuses on metabolic transitions between pre- and postprandial states during habitual daily activities and routine meals. Mass spectrometry-based metabolomics will be used to characterize metabolite profiles and their associations with diet, physical activity, sleep, and stress. In parallel, the study evaluates the feasibility of microsampling approaches, wearable-derived physiological data, and isotope-labelled standards for robust and cost-effective metabolite quantification. The results will inform the design of larger longitudinal studies using minimally invasive blood sampling in non-controlled settings.
Eligibility
Inclusion Criteria4
- Age: 18-65 years old
- Body Mass Index (BMI): 18.5-29.9 kg/m2
- Weight stability within the previous 3 months (self-reported).
- Positive intention to maintain usual routine physical activity and eating habits during the study.
Exclusion Criteria7
- Presence of acute chronic diseases, inflammatory or functional gastrointestinal disorders, or any diagnosed disease condition that disrupt daily routine activities.
- Routine use medications or supplements that could potentially interfere with the study outcomes
- History of eating disorder
- Recent use of antibiotic medication within the last 3 months prior to the first visit
- Active efforts to lose weight
- Smoking
- Consumption of alcoholic drinks exceeding nine standard cups per week and/or more than three standard cups per occasion
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Interventions
Each week, participants will eat one burger and collect finger blood before adn after consuming the burger 15 min, 30 min, 60 min, 120 min, 240 min
Finger-prick will be performed using a disposable pressure-activated lancet (Sterilance Press II, Vitrex; 21G, 2.8 mm incision) on which the lancet cannot be reactivated, and it returns to a safe plastic compartment after puncture. This mechanism reduces biohazard risks and risk of needlestick injuries. The finger-prick blood samples will be collected using a Capitainer quantitative dry blood spot (qDBS) micro sampling device (Capitainer). The total amount of blood obtained per time will be 20 - 100 µL. The micro sampling device has two inlet ports, and when a drop of blood encounters the inlet port of the device, blood flows by capillary forces in a microchannel and 10 -50 µL of blood is absorbed in a pre-perforated paper disk. This principle allows the collection of a fixed volume of blood and overcomes the hematocrit bias. Metabolite in microsamples will be extracted and analyzed using LC-MS platforms
An automatic glucose readings will be attached to participant to monitor daily glucose levels. Data will be acquired by the sensor and shared with the researcher
Locations(1)
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NCT07527793