Amino Acid Oxidation of Whole-Food Protein: a Dose-Response Crossover Trial in Young Adults
Maastricht University Medical Center
20 participants
Nov 1, 2025
INTERVENTIONAL
Summary
The purpose of this study is to assess the amino acid oxidation dose-response relationship of ingested whole-food protein in healthy adults. In a randomized, crossover design, 20 young, healthy adult males and females aged 18-35 years, will consume a dinner consisting of 0.32, 0.74, and 1.32 g protein･kg BM-1 (corresponding to 1, 2, and 4 minced beef patties, respectively) during 3 separate experimental trials.The main study endpoint is the amino acid oxidation of ingested protein over a 24-h period.
Eligibility
Inclusion Criteria4
- Aged between 18-35 years
- BMI between 18.5-30 kg/m2
- Healthy, recreationally active (exercise at least once per two weeks and a maximum of four days per week)
- No physical limitations (i.e., able to perform all activities associated with daily living independently)
Exclusion Criteria9
- Smoking
- Vegan, vegetarian, or does not consume beef protein
- Diagnosed musculoskeletal disorders
- Use of any medications known to affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications)
- Chronic use of gastric acid-suppressing medication or anti-coagulants
- Unstable weight over the last three months
- Diagnosed GI tract disorders or diseases
- Blood donation in the past 2 months
- Females: pregnancy
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Interventions
Dinner meal consisting of minced beef protein.
Locations(1)
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NCT07547176