RecruitingNot ApplicableNCT03704766

Alpha-Defensin and Synovial Proteins to Improve Detection of Pediatric Septic Arthritis


Sponsor

Hospital for Special Surgery, New York

Enrollment

442 participants

Start Date

Jun 28, 2016

Study Type

INTERVENTIONAL

Conditions

Summary

Differentiating between septic arthritis and other causes of joint inflammation in pediatric patients is challenging and of the utmost importance because septic arthritis requires surgical debridement as part of the treatment regimen. The current gold standard to diagnose septic arthritis in children is a positive synovial fluid culture; however, joint cultures may take several days to return. If a bacterial infection is present, it requires immediate surgical intervention in order to prevent lasting articular cartilage damage. Frequently surgeons must decide whether to surgically debride a joint before culture results are available. There is no single lab test or clinical feature that reliably indicates bacterial infection over other causes of joint inflammation. The alpha-defensin assay has shown high sensitivity and specificity for joint infection in other studies.The purpose of this study is to determine the sensitivity and specificity of several synovial biomarkers for diagnosing pediatric septic arthritis.


Eligibility

Max Age: 17 Years

Inclusion Criteria3

  • Synovial fluid is obtained to assess for infection or inflammatory/rheumatologic disease (all medium and large joints will be included: hip, knee, ankle, shoulder, subtalar, elbow, and wrist joints)
  • Patients with recent antibiotic exposure are eligible to participate but will be analyzed separately
  • Patients undergoing a procedure unrelated to infection (the procedure may be arthroscopy, or an open or percutaneous bony or soft tissue procedure)

Exclusion Criteria4

  • Family declines to participate/consent
  • Patients with a major joint trauma (such as a documented ligament tear or fracture) within the past 8 weeks are not eligible to have that joint aspirated, but could have another joint aspirated
  • A history of recent infection (within the past 3 months)
  • Received antibiotics in the past 7 days

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Interventions

DIAGNOSTIC_TESTSynovial Alpha-defensin assay

One of the synovial fluid (joint fluid) tests that will be performed by the outside lab, CD Diagnostics.

DIAGNOSTIC_TESTSynovial Neutrophil elastase assay

One of the synovial fluid (joint fluid) tests that will be performed by the outside lab, CD Diagnostics.

DIAGNOSTIC_TESTSynovial lactate assay

Synovial Fluid (joint fluid) will be sent out to CD Diagnostics for this test.

DIAGNOSTIC_TESTSynovial C-reactive Protein (CRP)

One of the synovial fluid (joint fluid) tests that will be performed by the outside lab, CD Diagnostics.

DIAGNOSTIC_TESTSynovial Staphylococcus spp antigen panel

One of the synovial fluid (joint fluid) tests that will be performed by the outside lab, CD Diagnostics.

DIAGNOSTIC_TESTSynovial Candida spp antigen panel

One of the synovial fluid (joint fluid) tests that will be performed by the outside lab, CD Diagnostics.

DIAGNOSTIC_TESTSynovial Enterococcus faecalis assay

One of the synovial fluid (joint fluid) tests that will be performed by the outside lab, CD Diagnostics.

DIAGNOSTIC_TESTSynovial bacterial culture by BacT/Alert

The synovial fluid (joint fluid) sent to CD Diagnostics for testing will also be cultured to see if any organisms grow.

DIAGNOSTIC_TESTSynovial Cell count + differential (CBC)

One of the synovial fluid (joint fluid) tests that will be performed by the outside lab, CD Diagnostics.

DIAGNOSTIC_TESTSynovial Gram Stain

This will be performed by CD Diagnostics if any organisms are present in the synovial fluid.

DIAGNOSTIC_TESTSynovial Leukocyte Esterase Test Strips

These test strips will be tested at the hospital where the patient is being seen, a few drops of synovial fluid will be used on the strip.

DIAGNOSTIC_TESTSynovial PCR for Kingella kingae

In participants \< 8 years old, a sample of synovial fluid will be tested for Kingella kingae using PCR.

DIAGNOSTIC_TESTSerum Cell count + differential (CBC)

A blood test performed at the hospital.

DIAGNOSTIC_TESTSerum erythrocyte sedimentation rate (ESR)

A blood test performed at the hospital.

DIAGNOSTIC_TESTSerum C-reactive Protein (CRP)

A blood test performed at the hospital.

DIAGNOSTIC_TESTSerum D-dimer

A blood test performed at the hospital.

DIAGNOSTIC_TESTSerum Procalcitonin

A blood test performed at the hospital.

DIAGNOSTIC_TESTBlood Cultures

Blood will be cultured at the hospital to see if any organisms grow.

DIAGNOSTIC_TESTOptional blood testing per standard of care (ASO, anti-strep, ANA, anti-DS-DNA, HLA-B27, RF, Lyme and other inflammatory/ rheumatologic markers )

In patients with suspected inflammation/infection, there are other blood tests which may be standard of care and used for diagnosis.


Locations(3)

Children's Healthcare of Atlanta

Atlanta, Georgia, United States

Hospital for Special Surgery

New York, New York, United States

Campbell Clinic

Collierville, Tennessee, United States

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NCT03704766


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