RecruitingNCT04899635

POWER Myocardial Fatigue Study: a Biomechanical Assessment of Contractility of Human Myocardium

Profiling Biomechanical Responses and Workload of the Human Myocardium to Explore the Concept of Myocardial Fatigue and Reversibility


Sponsor

University Hospitals Coventry and Warwickshire NHS Trust

Enrollment

100 participants

Start Date

Aug 3, 2021

Study Type

OBSERVATIONAL

Conditions

Summary

To gain a comprehensive understanding of the biomechanical behaviour of human heart to explore the concept of myocardial fatigue in response to a temporal range of preload, afterload and drug-induced inotropy using in-vitro contractile assays.


Eligibility

Min Age: 18 YearsMax Age: 85 Years

Inclusion Criteria2

  • All adult patients between 18 to 85-years old undergoing open-heart surgery who can undergo the consent process for the study
  • Healthy donor hearts that are deemed non-transplantable and consent received from a legal representative

Exclusion Criteria3

  • This criterion is kept to a minimum since the availability of human myocardial samples is finite and dependent on the limited number of patients undergoing cardiac surgery annually within the local hospital.
  • Patients who do not have the mental capacity to undergo the consent process
  • For the safety of researchers, patients with evidence of ongoing blood-borne infections such as HIV, or a recent positive test for COVID-19 (within 10 days of last PCR test).

Interventions

OTHERIn-vitro contractile fatigue protocol

Using contractility assays for muscle slices or the work-loop assay for isolated heart cells, the tissue preparation will undergo a series of contraction and relaxation under varying levels of preload, afterload, stimulation frequency and under other experimental conditions such as drug-induced inotropism.


Locations(1)

University Hospitals Coventry and Warwickshire

Coventry, United Kingdom

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NCT04899635


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