RecruitingPhase 1NCT05779917

Mesothelin/GPC3/GUCY2C-CAR-T Cells Against Cancers

Mesothelin/GPC3/GUCY2C Targeted CAR-T for Immunotherapy of Pancreatic Cancer: Phase I Clinical Trial


Sponsor

Second Affiliated Hospital of Guangzhou Medical University

Enrollment

30 participants

Start Date

Mar 10, 2023

Study Type

INTERVENTIONAL

Conditions

Summary

The second generation of mesothelin targeted CAR-T cells that secret a fusion protein of IL21 and scfv against PD1 have been constructed and their anti-cancer function has been verified by multiple in vitro and in vivo studies. Clinical studies will be performed to test anti-cancer function of the CAR-T cells for immunotherapy of human cancer patients with Mesothelin expressions. In this phase I study, the safety, tolerance, and preliminary efficacy of the Mesothelin-CAR-T cell immunotherapy on human cancers will firstly be evaluated.


Eligibility

Min Age: 18 YearsMax Age: 75 Years

Inclusion Criteria2

  • \. Patients with advanced cancer that expresses Mesothelin protein; 2. Life expectancy \>12 weeks; 3. Adequate heart, lung, liver, kidney, and blood function; 4. Available autologous transduced T cells with greater than or equal to 20% expression of Mesothelin-CAR determined by flow-cytometry and killing of Mesothelin-positive targets greater than or equal to 20% in cytotoxicity assay; 5. Informed consent explained to, understood by and signed by patient/guardian. Patient/guardian given copy of informed consent.
  • \-

Exclusion Criteria8

  • Had accepted gene therapy before;
  • Severe virus infection such as HBV, HCV, HIV, et al;
  • Known HIV positivity;
  • Active infectious disease related to bacteria, virus,fungi,et al;
  • Other severe diseases that the investigators consider not appropriate;
  • Pregnant or lactating women;
  • Systemic steroid treatment (greater than or equal to 0.5 mg prednisone equivalent/kg/day);
  • Other conditions that the investigators consider not appropriate. -

Interventions

BIOLOGICALCAR-T cells

Transfer CAR-T cells into patients for anti-ancer therapy.


Locations(1)

The Second Affiliated Hospital of Guangzhou Medical University

Guangzhou, Guangdong, China

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NCT05779917


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