RecruitingNot ApplicableNCT06023472

Microfluidic Chip vs Density Gradient Centrifugation on the Euploidy Rate of Pre-implantation Genetic Testing

A Randomized Comparison of Microfluidic Chip vs Density Gradient Centrifugation on the Euploidy Rate of Pre-implantation Genetic Testing

Sponsor

Professor Ernest Hung-Yu Ng

Enrollment

318 participants

Start Date

Nov 1, 2024

Study Type

INTERVENTIONAL

Conditions

Aneuploidy Infertility Genetic Disease Chromosomal Rearrangement

Summary

Infertile women attending for PGT at the Centre of Assisted Reproduction and Embryology, Queen Mary Hospital and Kwong Wah Hospital will be recruited during ovarian stimulation for IVF. Subsequently, they will be randomly assigned on the day of oocyte retrieval by a laboratory staff into one of the following two groups in a 1:1 ratio : (1) the microfluidic chip group and (2) the density gradient centrifugation group for sperm preparation and subsequent use in fertilization. Other IVF procedures will be the same as the standard practice of the Centre. Both women and clinicians will be blinded from the group allocation i.e. a double blind study.

Eligibility

Max Age: 43 Years

Plain Language Summary

Simplified for easier understanding

This clinical trial is studying a medical device called density gradient centrifugation and a medical device called microfluidic chip for people with aneuploidy, chromosomal rearrangement, and other related conditions. The study is currently recruiting participants at 1 location. People eligible for this study include up to age 43 Years.

This summary was AI-generated to explain the trial in plain language. It is not medical advice. Always discuss eligibility with your doctor before enrolling in a clinical trial.

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Interventions

DEVICEmicrofluidic chip

Microfluidic chip method has been used for sperm sorting in order to select the most motile and morphologically normal sperm for use in assisted reproductive technologies (ART) such as in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).

DEVICEdensity gradient centrifugation

Density-gradient centrifugation is a commonly used method for sperm separation and purification. It is a technique that involves layering a semen sample on top of a gradient of different densities of a solution, typically a mixture of colloidal silica and sucrose, and then centrifuging the sample. The centrifugal force causes the sperm to migrate through the gradient, where they become separated based on their density.