RecruitingNot ApplicableNCT06221787

Stem Cell Derived Exosomes in the Treatment of Melasma and Its Percutaneous Penetration


Sponsor

Fujian Medical University Union Hospital

Enrollment

80 participants

Start Date

Apr 1, 2022

Study Type

INTERVENTIONAL

Conditions

Summary

Melasma is a refractory skin disease due to its complex pathogenesis and difficult treatment. Studies have found that human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-Exos) could serve as a novel cell-free therapeutic strategy in regenerative and aesthetic medicine. It could potentially treat melasma, but the skin barrier is a challenge. This study aims to explore the safety and efficacy of hUCMSC-Exos in the treatment of melasma and means to promote its percutaneous penetration.


Eligibility

Min Age: 18 YearsMax Age: 60 Years

Inclusion Criteria5

  • \- Patients diagnosed with chloasma who meet the clinical diagnostic standards and efficacy standards (revised version) of chloasma and have skin lesions on the face.
  • It is hoped that exosomes combined with 1565 non-ablative fractional laser, blue aurora or micro-needle can improve facial chloasma.
  • Fully understand and understand the content and significance of this study, implementation plan, possible benefits, risks and countermeasures, rights and obligations of subjects (including privacy protection, free withdrawal), willing to participate in this clinical study and able to cooperate well, Those who signed the informed consent form.
  • Exclude post-inflammatory pigmentation, malar brown-green nevus, Riehl's melanosis, pigmented lichen planus and other skin diseases.
  • Subjects who agree not to use other cosmetic treatments related to the study during the study period.

Exclusion Criteria13

  • Patients who refuse to sign the informed consent form to participate in the trial;
  • Those with a history of important organ diseases, or a history of autoimmune diseases or immune dysfunction;
  • Abnormal coagulation function, current use of anticoagulants, thrombophilia and history of familial genetic diseases;
  • Pregnant or lactating women;
  • Patients taking oral contraceptives or hormone replacement therapy during the study period or within the past 12 months;
  • Patients with scar constitution;
  • Active skin infection;
  • Those who have a history of multiple severe allergies, a history of hereditary allergies, photosensitivity or photosensitivity drugs, such as sulfa drugs and tetracyclines, those who are allergic to local anesthetics and those who are allergic to lidocaine components, and those who plan to undergo detoxification during the study Allergy healer;
  • History of post-inflammatory pigmentation;
  • Those who have received treatment for chloasma in the past;
  • Those who have undergone chemical peeling, dermabrasion or other skin resurfacing on their face in the past;
  • Patients who are participating in other clinical studies;
  • Other reasons that the researcher considers unsuitable for clinical investigators.

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Interventions

PROCEDURE1565 nm non-ablative fractional laser combined with normal saline

After 1565 nm non-ablative fractional laser treatment, patients with melasma were smeared with normal saline and to compared with those smeared with exosomes after laser treatment.

PROCEDUREmicroneedles combined with hUCMSC-Exos

the hUCMSC-Exos were applied while rolling a microneedle roller and to compared with those used 1565 nm non-ablative fractional laser or PBASM combined with hUCMSC-Exos.

PROCEDURE1565 nm non-ablative fractional laser combined with hUCMSC-Exos

After 1565 nm non-ablative fractional laser treatment, patients with melasma were smeared with hUCMSC-Exos and to compared with those smeared with normal saline after laser treatment.What's more,compared with those used microneedles or PBASM combined with hUCMSC-Exos.

PROCEDUREPBASM combined with hUCMSC-Exos

a plasma named Peninsula Blue Aurora Shumin Master (PBASM) rolling each area for 8-10 min before applying the hUCMSC-Exos in the entire face. And to compared with those used 1565 nm non-ablative fractional laser or microneedles combined with hUCMSC-Exos.


Locations(1)

Affiliated Union Hospital of Fujian Medical University

Fuzhou, Fujian, China

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NCT06221787


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