RecruitingNot ApplicableNCT06302933

Negative Serology by Immunoenzymatic Test (EIA) in HIV-infected Children Treated Early With Antiretroviral in the ANRS-Pediacam Study: Pathophysiological Mechanisms


Sponsor

ANRS, Emerging Infectious Diseases

Enrollment

451 participants

Start Date

May 2, 2024

Study Type

INTERVENTIONAL

Conditions

Summary

The objective of the study is to identify the pathophysiological mechanisms responsible for the induction and maintenance of negative serologies by EIA tests in HIV-infected children treated early with HAART in the ANRS 12225-Pediacam III cohort in Cameroon The hypothesis of better control of HIV infection through interactions between immunological, viral, and genetic factors was made to build the following objectives: * Immunological aspect: lack of humoral response or immune activation * Virological aspect: Reduced HIV reservoir size * Determine the HLA phenotype in the different groups of children included and the KIR genotypes.


Eligibility

Inclusion Criteria10

  • Case control study
  • Children included and followed in the ANRS 12225 study - Pediacam III
  • Having plasma samples in the bio bank during the above-mentioned periods Case:children with at least one negative HIV serology made by ELISA, permanent or transientduring follow-up.
  • Control (4 groups)
  • HIV-infected children with positive serology and viral load (VL) \<400 copies /ml
  • HIV-infected children with positive serology and VL ≥400 copies / ml
  • HIV-uninfected children born to HIV-positive mothers
  • HIV-uninfected children born to HIV-uninfected mothers Selection of cases and controls will be matched on gestational age (premature \<37, term ≥37 weeks) and year of birth (2007-2008 and 2009-2010).
  • All children still followed in the ANRS - Pediacam III cohort
  • Written consent of one of the parents or the guardian and assent of the child if aged ≥ 11 years and complete disclosure of HIV statusfor infected children for participation to the study.

Exclusion Criteria2

  • Refusal by one of the parents or the guardian for the child's participation in the study
  • No assent of the child (if aged ≥ 11 years and with complete disclosure of HIV status, for infected children)

Interventions

BIOLOGICALBlood sampling

Blood samples collected from children followed in the Pediacam III ANRS12225 cohort


Locations(3)

Hôpital de Jour - Hôpital Laquintinie de Douala

Douala, Cameroon

Unité Pédiatrique de Jour - Centre Mère et Enfant de la Fondation Chantal Biya

Yaoundé, Cameroon

Service de Pédiatrie - Centre Hospitalier d'Essos

Yaoundé, Cameroon

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NCT06302933


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