RecruitingNot ApplicableNCT06466746

The Impact of Faba Bean Rich Bread on Iron Status, Postprandial Lipaemia and Satiety

The Impact of Faba Bean Rich Bread on Iron Status, Postprandial Lipaemia and Satiety in Adults with Low Iron Stores


Sponsor

University of Reading

Enrollment

16 participants

Start Date

Sep 1, 2024

Study Type

INTERVENTIONAL

Conditions

Summary

High levels of animal proteins (meat) in the diet are linked with a greater risk of developing heart disease and other long-term health conditions. Recently there has been a shift to plant-based diets including plant proteins such as pulses, defined as beans, peas, chickpeas and lentils. Pulses are a nutritious and sustainable form of plant protein which are rich in fibre and iron. Despite this, the UK population does not consume the recommended daily amount of pulses (80g/day equivalent to a large handful). In contrast, bread is commonly consumed but very little is known about how bread enriched with pulses influences the amount of iron that is digested and absorbed by the body as well as risk factors for developing heart disease and type 2 diabetes. The main purpose of this randomised controlled cross-over study is to determine how consuming bread enriched with pulses (in the form of faba bean flour at approximately 40% enrichment) compared with conventional white bread (100% wheat flour) influences the amount of iron absorbed in healthy males and females aged 18-50 years with low iron stores. Secondary aims are determining the effects on blood fats and sugar (glucose) and on feelings of fullness (also known as satiety) after eating the bread enriched in pulses and conventional white bread. Participants will be required: * To attend for seven study visits over a period of 60 days. * Consume a breakfast meal containing either the faba bean-enriched bread or conventional bread over two study periods, each consisting of one 7 hour study visit and two consecutive visits of 2 hours in duration. After 28 days, participants will return for the second study period as above, with a final study visit conducted 28 days later. * Give blood samples during 2 x 7 hour study visits * Complete visual analogue scales to rate appetite after consuming the faba bean-enriched and conventional white bread. * Record dietary intake prior to and during the study period.


Eligibility

Min Age: 18 YearsMax Age: 50 Years

Inclusion Criteria4

  • Non-anaemic, males and pre-menopausal females (must have regular periods)
  • Aged 18-50 years old
  • BMI between 19 - 30 kg/m2
  • Low iron stores (serum ferritin between 13-40 µg/L for females and 30-90 µg/L for males)

Exclusion Criteria16

  • Food allergies or intolerances to faba beans (favism) or gluten
  • Diagnosed with anaemia (haemoglobin \<115 g/L for females or \<130 g/L for males) or haemochromatosis
  • C-reactive protein (\>3mg/L)
  • Smokers
  • A history of alcohol abuse (\> 14 units/ week)
  • CVD or medical history of MI or stroke in the past 12 months
  • Diabetes (fasting glucose \>7.0 mmol/L)
  • Kidney, liver, pancreas or intestinal disease, gastrointestinal disorder or use of drugs likely to alter gastrointestinal function
  • Pregnancy, planning a pregnancy in the next six months or breastfeeding
  • Peri- and post-menopausal women or women with irregular periods
  • Planning on a weight-reducing regimen (lost \>3kg in last 6 months)
  • Parallel participation in another intervention study
  • Hypertension (blood pressure \> 140/90 mmHg), cancer, taking medication for hyperlipidaemia (statins), or inflammation
  • Taking vitamin or mineral supplements
  • Donated blood in the last 3 months
  • Any other unusual medical history or diet and lifestyle habits or practices that would preclude volunteers from participating in a dietary intervention and metabolic study (such as a pacemaker)

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Interventions

OTHERRaising the Pulse faba bean-enriched bread

100 g of white bread produced using 40% faba bean enriched flour and 60% wheat flour will be provided in the breakfast meal with stork margarine, chocolate spread, a glass of orange juice and a glass of water containing the iron-57 stable isotope. An ad-libitum lunch consisting of pasta and a tomato sauce will be provide 6 hours after the breakfast.

OTHERConventional White Bread

100 g of white bread produced using 100% wheat flour will be provided in the breakfast meal with stork margarine, chocolate spread, a glass of orange juice and a glass of water containing the iron-57 stable isotope. An ad-libitum lunch consisting of pasta and a tomato sauce will be provide 6 hours after the breakfast.


Locations(1)

Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading

Reading, Berkshire, United Kingdom

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NCT06466746


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