RecruitingNot ApplicableNCT06561971

Effect of Different Centrifuge Values on Saliva High Sensitive C-Reactive Protein (Hs-CRP) Levels in Individuals With Different Smoking Amounts

Evaluating the Effect of Smoking Amount on Determining Salivary High Sensitive C-Reactive Protein(Hs-CRP) Values by Centrifuging the Saliva of the Same Individuals at Different Rpm and Time.

Sponsor

Izmir Katip Celebi University

Enrollment

90 participants

Start Date

Jun 1, 2024

Study Type

INTERVENTIONAL

Conditions

Smoking

Summary

The purpose of this study; In periodontally healthy individuals, high sensitive c-reactive protein (hs-CRP) levels of non-smokers, smokers of less than 10 cigarettes per day and smokers of more than 10 cigarettes per day were measured at 15 rpm-10 min, 10 centrifuge values, which are frequently used in saliva studies. To determine the levels in saliva samples as a result of rpm-10 min and 10 rpm 6 min centrifugations and to determine the values at which a cytokine of this molecular weight should be centrifuged and to examine the possible correlation between these values and the clinical parameters of the amount of smoking. Materials and Methods: saliva samples were collected from 90 systemically healthy individuals who were non-smokers (N, n=30), light smokers \<10 cigarettes per day (L, n=30) and heavy smokers \>10 cigarettes per day (H, n=20). samples were taken. Full-mouth clinical periodontal measurements, including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI), and plaque index (PI), were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine hs-CRP levels in biological samples.

Eligibility

Min Age: 18 YearsMax Age: 30 Years

Inclusion Criteria10

Systemically healthy
Periodontally clinical healthy
At least 20 permanent teeth in the mouth
No medication for continuous use
Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
Not in pregnancy or lactation period.
Not received periodontal treatment in the last 6 months
For the non-smoker group; person who does not smoke tobacco
For the light-smoker group; person who smokes less than 10 cigarettes a day
For heavy-smoker group; person who smokes more than 10 cigarettes a day

Exclusion Criteria6

Any oral or systemic disease
Regularly using a systemic medication
During pregnancy or lactation
Received periodontal treatment within the last 6 months.
Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
Individual with periodontal disease

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Interventions

DIAGNOSTIC_TESTsaliva obtaining

The patient was asked to sit upright and tilt his/her head forward to collect saliva samples. İn this way, unstimulated saliva was allowed to accumulate in the floor of the mouth. The accumulated saliva was collected in a sterile container. It was then transferred to a propylene tube. The tubes were centrifuged and the clear part at the top of the tube was taken with a sterile syringe and transferred to a different propylene tube with 0.5 ml in each tube. Tubes were stored at -80ºC until the day of analysis.