RecruitingPhase 4NCT06739473

Total-body Glucose Utilization in Obesity


Sponsor

Turku University Hospital

Enrollment

60 participants

Start Date

Jan 25, 2023

Study Type

INTERVENTIONAL

Conditions

Summary

There are three goals for this study. One is to investigate how different emotions and exposure to cold affect blood flow in the body. The second aim is to study how insulin affects blood flow and tissue glucose uptake, and to study whether this effect is altered in obesity. Last, the study focuses on establishing the lowest dose of a PET radiotracer, oxygen-15 labelled water, that can be used in clinical studies with a new whole-body PET scanner.


Eligibility

Min Age: 20 YearsMax Age: 60 Years

Inclusion Criteria3

  • Age 20-60 years
  • BMI either 18.5-25 kg/m2 or above 30 kg/m2
  • Use of hormonal contraception for women in child bearing age

Exclusion Criteria9

  • In the lean group history of obesity or type 2 diabetes
  • In the group with obesity: current or previous use of GLP-1-agonists or insulin
  • History of eating disorders
  • Smoking, use of narcotics, excessive use of alcohol
  • Previous participation in PET studies
  • Previous significant exposure to radiation
  • Claustrophobia
  • Active plans for concieving (both sexes)
  • Any other condition that in the opinion of the investigator could create a hazard to the subject safety, endanger the study procedures or interfere with the interpretation of study results

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Interventions

BEHAVIORALMovies

Participants will watch eight short movie clips, and the changes different emotions evoked by the clips will be studied using H2\[15O\]O PET/CT.

BEHAVIORALCold

Participants will be studied with H2\[15O\]O PET/CT while one of their legs will be exposed to cold to study the effects of cold stimulus on whole-body blood flow.

DRUGHyperinsulinemic euglycemic clamp

Participants will be administered intravenous fast acting insulin (NovoRapid, Novo Nordisk, 1 mU/m2 body surface area/min) together with 20 % glucose to maintain normal glucose values (5.0 mmol/L) to study the effects of insulin on whole body blood flow with H2\[15O\]O PET/CT, and to assess tissue insulin-stimulated glucose uptake with \[18F\]FDG PET/CT.

RADIATIONH2[15O]O PET/CT

H2\[15O\]O PET/CT, with a dose of 25-700 MB/bolus repeated 2-8 times per scan.

RADIATION[18F]FDG PET/CT

Whole-body \[18F\]FDG PET/CT scan to study tissue glucose uptake, dose 100 MBq

DRUGCEUS or Contrast enhanced Ultrasound

Contrast-enhanced ultrasound of left forearm muscles is performed during fasting and after 30-40 minutes of hyperinsulinemia


Locations(1)

Turku PET Centre

Turku, Finland

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NCT06739473


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