RecruitingNot ApplicableNCT06849622

Auto-antibody Dosage From Blood Spots for Diagnosis of Type 1 Diabetes and Celiace Disease


Sponsor

Meyer Children's Hospital IRCCS

Enrollment

1,500 participants

Start Date

Nov 4, 2024

Study Type

INTERVENTIONAL

Conditions

Summary

Early diagnosis of type 1 diabetes and celiac disease is very useful, allows early therapy and prevents deaths from the onset of diabetic ketoacidosis. This is a pilot study on screening of autoantibodies of type 1 diabetes and celiac disease in tuscany patients. The study aims to evaluate the concordance between the screening results obtained using two different matrix (blood drop spots on card and serum) in the search for autoantibodies for celiac disease and for type 1 diabetes. Moreover, it will be evaluated the feasibility and acceptability of the screening on a sample of the population enrolled in the territory through the participation of pediatricians.


Eligibility

Min Age: 2 YearsMax Age: 13 Years

Inclusion Criteria3

  • Patients aged 2-13 years
  • Patients with a confirmed diagnosis of type 1 diabetes or celiac disease (positive controls) and children who do not have type 1 diabetes or celiac disease or autoantibodies associated with these pathologies (controls)
  • Obtained informed consent

Exclusion Criteria1

  • none

Interventions

DIAGNOSTIC_TESTTest on blood sample

The blood sample will be drawn by venipuncture during clinical routine. The serum will be tested for coeliac or diabetes 1 auto-antibodies, using Elisa test.

DIAGNOSTIC_TESTTest on dried blood sample

A finger prick will be used to collect Dried blood Spot (DBS) on a special card. The eluted blood spot will be tested, for coeliac and diabetes 1 auto-antibodies, using the same method used for serum, and the same cut-offs for positivity and negativity (Elisa tests)


Locations(1)

Meyer Children's Hospital IRCCS

Florence, Italy, Italy

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NCT06849622


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