RecruitingNCT07060716

Validation of Donor-Derived Cell-Free DNA (Dd-cfDNA) for Kidney Transplant Monitoring

GraftAssureDx Study: Validation of Donor-Derived Cell-Free DNA (Dd-cfDNA) for Kidney Transplant Monitoring


Sponsor

Insight Molecular Diagnostics

Enrollment

125 participants

Start Date

Sep 8, 2025

Study Type

OBSERVATIONAL

Conditions

Summary

The goal of this observational study is to learn if the donor-derived cell-free DNA (dd-cfDNA) test can assess rejection in kidney transplant recipients. Participants will have blood and urine collected at their study visit. Researchers will compare results of the GraftAssureDx to rejection detected by standard-of-care graft biopsies.


Eligibility

Min Age: 18 Years

Inclusion Criteria6

  • Subject is 18 years of age or older
  • At least 12 calendar days have elapsed since the subject received a kidney transplant.
  • Subject has provided legally effective informed consent
  • Subject agrees to comply with all study procedures
  • \. A graft biopsy is obtained within ±1 week of blood draw.
  • If blood draw is obtained after biopsy, blood draw should be taken at least 2 days after an uncomplicated biopsy procedure.

Exclusion Criteria10

  • Kidney donor is an identical twin of the subject.
  • The subject has another previously transplanted organ in situ.
  • Subject has received a hematopoietic stem cell transplant.
  • Subject has received a bone marrow graft.
  • Subject has self-reported as pregnant.
  • In the opinion of the investigator, the subject's participation in the study would pose a risk to data integrity or to the subject's safety and welfare.
  • Sample collected from someone that had an invasive graft biopsy ≤ 48 hours prior to blood draw.
  • Sample collected from subject that received immunosuppressive treatment for biopsy-proven acute rejection ≤ 30 days prior to blood draw
  • Sample collected from subject that received a blood transfusion ≤ 30 days prior to blood draw.
  • Sample collected from a subject that provided another sample for the study within the past 7 days.

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Interventions

DIAGNOSTIC_TESTdonor-derived cell-free DNA test

A donor-derived cell-free DNA (dd-cfDNA) test used to measure the concentration of total cell-free DNA and the fractional abundance of the dd-cfDNA.


Locations(10)

University of Southern California Keck School of Medicine

Los Angeles, California, United States

Mayo Clinic in Florida

Jacksonville, Florida, United States

Tampa General Hospital

Tampa, Florida, United States

Cleveland Clinic

Cleveland, Ohio, United States

University of Pittsburgh Medical Center

Pittsburgh, Pennsylvania, United States

Vanderbilt University Medical Center

Nashville, Tennessee, United States

Baylor, Scott & White Research Institute

Dallas, Texas, United States

Intermountain Health

Murray, Utah, United States

Institute of Immunology - Transplantation Immunology

Heidelberg, Baden-Wurttemberg, Germany

Charite Universitatsmedizin

Berlin, Germany

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NCT07060716


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