Evaluating the Utility of Implementing Microfluids for Sperm Preparation Compared to Conventional Method of Density Gradient Centrifugation in a PGT-A Program: a Sibling Oocyte Study
ART Fertility Clinics LLC
100 participants
Sep 12, 2025
INTERVENTIONAL
Conditions
Summary
In assisted reproductive technology (ART), sperm preparation aims to select the most viable sperm for ICSI. Unlike conventional methods like density gradients or sperm washing, microfluidic techniques mimic natural selection in the female reproductive tract by using laminar flow without centrifugation, reducing the risk of DNA damage. This method isolates highly motile sperm while filtering out debris and immotile cells. Studies show that microfluidics improve embryo quality, increase pregnancy rates, and may lead to higher euploidy rates. Additional benefits include improved safety, scalability, and shorter preparation times.
Eligibility
Inclusion Criteria5
- Women with at least 8 MII per cycle after denudation (AFC≥8).
- Women of all ages.
- All embryo qualities ≥BL3CC at the time of biopsy on day 5, 6 and/or 7.
- Fresh sperm used from ejaculate with a concentration ≥1 mill/ml and ≥10% motility (A+B).
- Sperm samples with a minimum of 2 ml.
Exclusion Criteria3
- Frozen oocytes samples with severe oligospermia (≤1mill/ml).
- PGT-M cases
- Sperm with \> 1M/ml of round cells
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Interventions
The FERTILE PLUS™ method is a standardized method with an easy-to-follow protocol that is far less dependent on the skill or experience of the embryologist than other methods, such as density gradients. The FERTILE PLUS™ (850 µL) Sperm Sorting Chip is a single-use, flow-free, dual chambered, microfluidic-based sperm sorting device. FERTILE PLUS™ was previously known as Zymot, prior to a name change by the manufacturer. The lower chamber contains a sample inlet and fluid channel separated from the upper collection chamber by a microporous membrane with 8-μm pores, demonstrated as the optimal size for selection of sperm with higher motility and normal morphology \[18\].
Locations(1)
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NCT07093619