RecruitingNCT07346833

Role of Circulating MicroRNAs in Differentiating Psychological Disorders Among Women With Chronic Toxoplasmosis

Deciphering The Role of Circulating MicroRNAs To Differentiate Psychological Disorders Among Women With Chronic Toxoplasmosis


Sponsor

Benha University

Enrollment

55 participants

Start Date

Nov 20, 2025

Study Type

OBSERVATIONAL

Conditions

Summary

Chronic Toxoplasma gondii (T. gondii) infection is a widespread condition that can negatively affect brain function and is considered a risk factor for various psychiatric conditions, including depression and schizophrenia. This prospective observational study aims to investigate the expression levels of specific circulating microRNAs (miRNAs) in women diagnosed with schizophrenia, major depressive disorder, or bipolar disorder who also have chronic toxoplasmosis. By comparing these levels to patients without the infection, the study seeks to determine if these miRNAs can serve as biological markers to help differentiate between specific psychological disorders and identify the impact of chronic toxoplasmosis on mental health.


Eligibility

Sex: FEMALEMin Age: 18 YearsMax Age: 50 Years

Inclusion Criteria3

  • Confirmed psychiatric diagnosis of Schizophrenia (SCZ), Major Depressive Disorder (MDD), or Bipolar Disorder (BD) according to DSM-5 criteria.
  • Seronegative for T. gondii IgM (to ensure infection is not in the acute phase).
  • Signed informed consent.

Exclusion Criteria3

  • Psychiatric disorders other than Schizophrenia, MDD, and Bipolar Disorder.
  • Seropositive for T. gondii IgM (indicating acute infection).
  • Refusal to participate.

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Interventions

DIAGNOSTIC_TESTCirculating miRNA Expression and T. gondii Serology

Serology: ELISA testing to detect anti-Toxoplasma IgG (confirming chronic infection) and IgM (excluding acute infection). Genetic Analysis: Quantitative Reverse-transcriptase PCR (qRT-PCR) to measure the expression levels of a predetermined panel of plasma miRNAs. Psychiatric Evaluation: Clinical diagnosis according to DSM-5 criteria.


Locations(1)

Benha faculty of Medicine

Banhā, El Qalyoubia, Egypt

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