RecruitingPhase 2NCT07381738

Unrelated Cord Blood Transplantation for EBV-associated Lymphoproliferative Disorders

A Single-arm, Two-center, Prospective Clinical Study of Unrelated Cord Blood Transplantation for EBV-associated Lymphoproliferative Disorders


Sponsor

Fudan University

Enrollment

80 participants

Start Date

Jan 1, 2026

Study Type

INTERVENTIONAL

Conditions

Summary

This clinical trial is designed as a single-arm, two-center, open-label, prospective study. All participants are enrolled in the standard-dose unrelated umbilical cord blood transplantation group to evaluate the efficacy and safety of unrelated umbilical cord blood transplantation in treating Epstein-Barr virus-associated lymphoproliferative disease.


Eligibility

Min Age: 14 YearsMax Age: 80 Years

Inclusion Criteria29

  • All subjects must meet all of the following criteria:
  • Age range: 14 to 80 years old, inclusive.
  • Diagnosed with an EBV-associated lymphoproliferative disease (including Chronic Active EBV Infection, EBV-associated Hemophagocytic Lymphohistiocytosis, and EBV-associated Lymphoma).
  • Meet at least one of the following disease-specific criteria (see below).
  • ECOG Performance Status score of 0-2 (or Lansky Play-Performance score ≥60 for children).
  • Expected survival of ≥3 months.
  • Signed informed consent form (for minors, consent must be provided by a legal guardian).
  • A. CAEBV Patients: Patients diagnosed with CAEBV according to the 2016 revised WHO classification criteria, who also meet all of the following:
  • Persistent or recurrent infectious mononucleosis-like symptoms for more than 3 months.
  • Elevated EBV-DNA load in peripheral blood or involved tissues (peripheral blood EBV-DNA \> 500 copies/ml).
  • Detection of EBV in T or NK cells (via EBER in situ hybridization or LMP1 immunohistochemistry).
  • Exclusion of other known immunodeficiency disorders, autoimmune diseases, or neoplastic diseases.
  • B. EBV-HLH Patients: Meet ≥5 of the HLH-2004 diagnostic criteria:
  • Persistent or recurrent infectious mononucleosis-like symptoms for more than 3 months.
  • Fever ≥38.5°C.
  • Splenomegaly.
  • Cytopenias (affecting ≥2 of 3 lineages):
  • Hb \<90 g/L
  • PLT \<100 × 10⁹/L
  • ANC \<1.0 × 10⁹/L
  • Hypertriglyceridemia (\>3 mmol/L) and/or hypofibrinogenemia (\<1.5 g/L).
  • Serum ferritin \>500 μg/L.
  • sCD25 \>2400 U/mL.
  • Decreased or absent NK cell activity.
  • Evidence of hemophagocytosis in bone marrow, spleen, or lymph nodes.
  • C. EBV-associated Lymphoma Patients:
  • Pathologically confirmed diagnosis according to WHO criteria.
  • EBV-positive (EBER+).
  • Failure of or relapse after first-line therapy.

Exclusion Criteria18

  • History of another primary malignancy within 5 years prior to the first treatment.
  • Uncorrected thyroid dysfunction.
  • Grade II or higher cardiac disease according to the New York Heart Association (NYHA) classification.
  • History of organ transplantation.
  • Planned to receive other types of hematopoietic stem cell transplantation during the study period.
  • Active infections, including:
  • Active hepatitis B or hepatitis C.
  • Positive serum HIV antigen or antibody.
  • History of syphilis.
  • Major surgery within 4 weeks prior to the first treatment, or anticipated need for major surgery during the study.
  • Pregnant or breastfeeding women.
  • History of severe psychiatric illness or substance abuse.
  • High risk of complications, such as:
  • Uncontrolled infection.
  • Active major visceral hemorrhage.
  • Known allergy to any component of the investigational drug(s), or a history of severe allergies.
  • Patients deemed unable to comply with the study procedures and/or follow-up requirements.
  • \-

Interventions

PROCEDUREUmbilical cord blood transplantation as treatment of Epstein-Barr virus-associated lymphoproliferative diseases

All participants will be enrolled in the treatment group and will receive a standard-dose unrelated cord blood transplantation. The efficacy and safety of unrelated cord blood transplantation for the treatment of Epstein-Barr virus-associated lymphoproliferative diseases will be evaluated.


Locations(2)

Huadong hospital, Fudan university

Shanghai, Shanghai Municipality, China

Huashan Hospital, Fudan University

Shanghai, Shanghai Municipality, China

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NCT07381738


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