RecruitingNot ApplicableNCT07671326

Antibacterial Efficacy of Bioceramic and Calcium Hydroxide Intracanal Medicaments

Antibacterial Efficacy of Bioceramic and Calcium Hydroxide-Based Intracanal Medicaments in Endodontic Treatment

Sponsor

Medipol University

Enrollment

24 participants

Start Date

Oct 15, 2025

Study Type

INTERVENTIONAL

Conditions

Root Canal Infection Periapical Periodontitis Intracanal Medicaments

Summary

This study evaluates the antibacterial effectiveness of two different intracanal medicaments, Bio-C Temp and UltraCal XS , in mandibular molar teeth with chronic apical periodontitis. Intracanal bacterial load is assessed before and after root canal preparation, after intracanal medicament removal, and after final irrigation using droplet digital PCR.

Eligibility

Min Age: 18 YearsMax Age: 65 Years

Inclusion Criteria4

Patients aged between 18 and 65 years
Being systemically healthy (ASA I or ASA II)
Mandibular molar teeth with a radiographically detectable radiolucent lesion in the periapical region
Willingness of the volunteer or their legal representative to participate in the study by signing the Informed Consent Form

Exclusion Criteria6

Patients who have received antibiotics or corticosteroids within the last 3 months
Patients with systemic diseases
Teeth that cannot be properly isolated with a rubber dam
Teeth with periodontal pockets >4 mm and teeth with crown/root fractures
Individuals who are undergoing or planning to start orthodontic treatment during the study period
Pregnant or breastfeeding individuals

Interested in this trial?

Get notified about updates and connect with the research team.

Interventions

PROCEDURERoot canal treatment with Bio-C Temp, a calcium silicate-based bioceramic intracanal medicament

Participants in this group undergo root canal shaping using VDW Rotate heat-treated nickel-titanium rotary files, and root canal preparation is completed up to #35/.04. The irrigation protocol includes 2.5% sodium hypochlorite (NaOCl) and 17% EDTA, followed by passive ultrasonic irrigation (PUI) using a VDW Ultra device with an IRRI S 21/25 ultrasonic tip. After chemomechanical preparation and microbiological sampling, Bio-C Temp, a calcium silicate-based bioceramic intracanal medicament, is placed into the root canals 1-2 mm short of the working length. The access cavity is temporarily sealed, and the medicament is left in place for 14 days.

PROCEDURERoot canal treatment with UltraCal XS, a calcium hydroxide-based intracanal medicament

Participants in this group undergo the same standardized root canal shaping and irrigation protocol as the Bio-C Temp group. Root canal shaping is performed using VDW Rotate heat-treated nickel-titanium rotary files and completed up to #35/.04. The irrigation protocol includes 2.5% sodium hypochlorite (NaOCl), 17% EDTA, and passive ultrasonic irrigation (PUI) with a VDW Ultra device and an IRRI S 21/25 ultrasonic tip. After chemomechanical preparation and microbiological sampling, UltraCal XS, a calcium hydroxide-based intracanal medicament, is placed into the root canals 1-2 mm short of the working length. The access cavity is temporarily sealed, and the medicament is left in place for 14 days.