S-Nitrosylation (SNO) Therapy During Autologous Blood Transfusion

This study investigates a new way to treat blood before it is re-infused back into a donor — a process called autologous blood transfusion. Blood stored outside the body loses some of its ability to deliver oxygen efficiently. The researchers believe that adding a specific chemical modification (S-nitrosylation) to the hemoglobin in stored blood can restore its oxygen-delivery function. This could be important for patients who donate their own blood before surgery and have it returned. Healthy volunteers will donate blood, which will be modified using the study compound and then re-transfused back into the same person. Researchers will closely monitor how the treated blood performs in delivering oxygen compared to unmodified stored blood. You may be eligible if: - You are a healthy adult aged 18–35 - Your hemoglobin is above 12 g/dL - You have no chronic diseases or pre-existing blood disorders - You are not pregnant or breastfeeding You may NOT be eligible if: - You are pregnant, breastfeeding, or unwilling to avoid pregnancy during the study - You have chronic heart, lung, kidney, or liver disease - You are taking anticoagulants (warfarin, heparin, etc.), certain antidepressants, beta-blockers, or nitrates - You have high blood pressure (above 130/90 mmHg) or abnormal heart rate - You have a blood clotting disorder or hemoglobin condition such as sickle cell - You are on long-term antihistamine therapy Talk to your doctor to see if this trial is right for you.