RecruitingNot ApplicableNCT04122456

DNA Repair Activity in the Skin of Day and Night Shift Workers

Pilot Study on DNA Repair Activity in the Skin of Day and Night Shift Workers


Sponsor

Wright State University

Enrollment

48 participants

Start Date

Dec 7, 2022

Study Type

INTERVENTIONAL

Conditions

Summary

The purpose of this study is to test how the skin of night shift workers responds to artificial sunlight (ultraviolet B radiation; UVB) at two different times of the day in comparison to normal day shift workers. After the skin biopsies are obtained, they will be brought to the laboratory to be exposed to UVB radiation and to measure UVB responses.


Eligibility

Min Age: 18 YearsMax Age: 40 Years

Inclusion Criteria5

  • Male/Female
  • Fair skin (Fitzpatrick types I and II)
  • Age 18 to 40
  • Able to comprehend procedures/risks
  • Primarily work and are awake during normal daylight hours (6 am to 6 pm), or primarily work and are awake during night shifts hours (between the hours of 6 pm and 6 am) or may work a mixture of these shifts over the past 3 months. Rotating shifts will be categorized based on the majority of hours worked on average during the last 3 months. Able to fill out a 3-month calendar of work schedule.

Exclusion Criteria11

  • Known photosensitivity
  • Currently on photosensitizing medications
  • Diabetes Mellitus
  • On any hormonal agents (e.g., birth control pills)
  • History of abnormal scarring
  • History of skin infections
  • History of skin cancers
  • History of sleep disorders such as sleep apnea or insomnia
  • Known allergy to lidocaine local anesthetic
  • Pregnancy or nursing
  • Other serious health issues

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Interventions

RADIATIONUltraviolet B Radiation

One skin punch biopsy will be taken to the laboratory for exposure to ultraviolet B radiation. After 1-hour incubation, the biopsies will be bisected in half.

OTHERNo Ultraviolet B Radiation

One skin punch biopsy will be taken to the laboratory and will be kept as a non-irradiated control.


Locations(1)

Wright State Physicians

Fairborn, Ohio, United States

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NCT04122456


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