RecruitingNCT06498336

miRNA in Septic Acute Kidney Injury

Specific miRNA Associated With Endothelial Dysfunction and Mitochondrial Damage in Patients With Septic Acute Kidney Injury


Sponsor

University Hospital Ostrava

Enrollment

220 participants

Start Date

May 2, 2024

Study Type

OBSERVATIONAL

Conditions

Summary

Acute kidney injury is a common and serious complication of sepsis and septic shock, which may be associated with a worse outcome of the patient's condition. The exact pathophysiological mechanism of septic acute kidney injury remains a challenge. One of the possible causes appears to be endothelial dysfunction and mitochondrial damage of renal tubular cells. The aim of this study is to identify specific microRNAs associated with these pathophysiological events in sepsis and septic acute kidney injury. And to establish a new potential diagnostic or therapeutic target for the prevention or treatment of septic acute kidney injury.


Eligibility

Min Age: 18 YearsMax Age: 100 Years

Inclusion Criteria7

  • The study group of patients with sepsis with or without acute kidney injury
  • age \> 18 years
  • newly diagnosed sepsis or septic shock with or without acute kidney injury
  • Healthy volunteers
  • no evidence of infection on clinical or laboratory examination
  • age \> 18 years
  • signed informed consent

Exclusion Criteria7

  • The study group of patients with sepsis with or without acute kidney injury
  • age \< 18 years
  • chronic kidney disease at stage 4 or 5 according to KDIGO recommendations (KDIGO) for chronic kidney disease 2024
  • patients on chronic dialysis treatment, or after renal transplantation
  • Healthy volunteers
  • acute or chronic infection
  • renal disease

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Interventions

DIAGNOSTIC_TESTLaboratory examination to determine the presence of sepsis

Standard laboratory biochemical methods will be used to determine sepsis diagnosis accord-ing to SEPSIS 3 (1). Blood samples for obtaining serum/plasma of the patients will be taken on the 1st and 4th day of sepsis diagnosis.

DIAGNOSTIC_TESTLaboratory examination to determine the presence of sepsis and acute kidney injury

Standard laboratory biochemical methods will be used to determine sepsis diagnosis according to SEPSIS 3 and acute kidney injury according to Guidelines Kidney Disease Improving Global Outcomes (KDIGO) 2012 (2). Blood samples for obtaining serum/plasma of the patients will be taken on the 1st and 4th day of sepsis diagnosis.

DIAGNOSTIC_TESTLaboratory examination to determine miRNA and biochemical parameters

Laboratory examination will be performed to determine miRNA and biochemical parameters.


Locations(2)

University Hospital Ostrava

Ostrava, Moravian-Silesian Region, Czechia

University Hospital Olomouc

Olomouc, Olomouc Region, Czechia

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NCT06498336


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