RecruitingNCT06782984

Drug Response Testing and Repurposing Using Glioblastoma Organoid

Patient-derived Organoids As Predictive Models for Drug Response Testing and Repurporsing in Glioblastoma Therapy


Sponsor

Chungnam National University Hospital

Enrollment

150 participants

Start Date

Aug 18, 2021

Study Type

OBSERVATIONAL

Conditions

Summary

The aim of this observational study, based on a prospectively collected cohort, is to evaluate the prognostic value of patient-derived organoids in predicting responses to conventional and repurposing drugs, including temozolomide, in patients with primary or recurrent glioblastoma. The primary question is whether the patient's response to temozolomide is recapitulated in their corresponding patient-derived glioblastoma organoid (GBO). Patient drug responses are evaluated using survival data, while GBO drug responses are assessed through a drug-response testing platform utilizing cell viability assays. Additionally, this platform is used to explore the potential application of various chemotherapeutic agents.


Eligibility

Plain Language Summary

Simplified for easier understanding

This study is using laboratory-grown mini tumor models (called organoids) made from a patient's own glioblastoma — the most aggressive type of brain tumor — to test which drugs work best for that individual. The aim is to find existing approved drugs that could be repurposed to treat each patient's specific tumor. **You may be eligible if...** - You have been diagnosed with glioblastoma (primary or recurrent) - You received standard treatment including surgery and temozolomide-based chemoradiation - Sufficient tumor tissue was collected during your surgery for organoid growth **You may NOT be eligible if...** - You did not undergo concurrent chemoradiation therapy following surgery - Post-treatment MRI was not performed - You prefer not to participate Talk to your doctor to see if this trial is right for you.

This summary was AI-generated to explain the trial in plain language. It is not medical advice. Always discuss eligibility with your doctor before enrolling in a clinical trial.

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Interventions

OTHEROrganoid-based drug sensitivity test

The intervention in this study involves utilizing glioblastoma organoids (GBOs) to perform an organoid-based drug sensitivity test (DST) and retrospectively comparing the results with clinical outcomes. Notably, no interventions will be applied to participants based on GBO-DST results; the study is limited to retrospective analysis. The GBO-DST is conducted by performing a drug response assay with temozolomide to determine the half-maximal inhibitory concentration (IC50), which serves to classify GBOs as TMZ-sensitive or TMZ-resistant. This classification is further validated through GBO cell survival analysis.


Locations(1)

Chungnam National University Hospital

Daejeon, Chungcheongnam-do, South Korea

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NCT06782984


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