Alterations in Mast Cell and Macrophage Infiltration, as Well as Micro Vessel Density
Understanding Alterations in Mast Cell and Macrophage Infiltration, as Well as Micro Vessel Density, May Throw Light on the Early Events Leading to Gastric Carcinogenesis in Obesity
General Committee of Teaching Hospitals and Institutes, Egypt
100 participants
Apr 15, 2026
OBSERVATIONAL
Conditions
Summary
Obesity is a global health problem that has reached epidemic proportions, affecting more than one billion people worldwide and significantly increasing the risk of multiple comorbidities, including type 2 diabetes, cardiovascular diseases, and cancer (World Health Organization, 2024). Increasing evidence suggests that chronic low-grade inflammation associated with obesity plays a critical role in the development of obesity-related malignancies, including gastric cancer. Adipose tissue dysfunction in obesity leads to the recruitment and activation of various immune cells, such as macrophages and mast cells, which contribute to a pro-inflammatory microenvironment through the release of cytokines, growth factors, and angiogenic mediators.
Eligibility
Inclusion Criteria3
- Adult patients undergoing bariatric surgery (laparoscopic sleeve gastrectomy).
- BMI > 35 kg/m²
- All participants underwent preoperative evaluation, including blood tests and assessment by a multidisciplinary team (nutritionist, psychiatrist, endocrinologist, radiologist, anesthesiologist, and surgeon).
Exclusion Criteria5
- Patients with secondary causes of obesity, such as Cushing's syndrome or polycystic ovary syndrome (PCOS).
- Patients with malignant gastric conditions or previous gastric surgery.
- Patients with systemic inflammatory diseases, autoimmune disorders, or chronic infections that may influence immune cell infiltration.
- Patients with incomplete clinical data or poor-quality tissue samples.
- Patients taking anti-inflammatory, immunosuppressive, or corticosteroid therapy within the last 3 months before sampling.
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Interventions
Adipose tissue macrophages (ATMs), mast cells positive for tryptase (MCPT), and microvascular density (MVD) were assessed by immunohistochemistry. Quantitative assessment was performed using a light microscope. For each GTO and NT tissue section, five highly immunostained areas ("hot spots") were identified at low magnification.
Adipose tissue macrophages (ATMs), mast cells positive for tryptase (MCPT), and microvascular density (MVD) were assessed by immunohistochemistry. Quantitative assessment was performed using a light microscope. For each GTO and NT tissue section, five highly immunostained areas ("hot spots") were identified at low magnification.
Locations(1)
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NCT07448896