RecruitingNot ApplicableNCT06068075

Liquid Biopsy in Ewing Sarcoma and Osteosarcoma as a Prognostic And Response Diagnostic: LEOPARD

Liquid Biopsy in Ewing Sarcoma and Osteosarcoma as a Prognostic And Response Diagnostic: The LEOPARD Study


Sponsor

Dana-Farber Cancer Institute

Enrollment

340 participants

Start Date

May 8, 2018

Study Type

INTERVENTIONAL

Conditions

Summary

This is a prospective multicenter biomarker study evaluating the prognostic impact of ctDNA detection at diagnosis in patients with Ewing sarcoma or osteosarcoma.


Eligibility

Min Age: 12 MonthsMax Age: 50 Years

Inclusion Criteria16

  • For Part A, subjects must meet all of the following eligibility criteria.
  • Age: ≥ 12 months of age at time of study enrollment to 50 years of age
  • Diagnosis: Patients with histologic diagnosis (by institutional pathologist) of newly diagnosed, localized or regionally disseminated Ewing sarcoma or peripheral primitive neuroectodermal tumor (PNET) of bone or soft tissue or; Patients with histologic diagnosis (by institutional pathologist) of newly diagnosed, non-pelvic, localized or regionally disseminated high-grade osteosarcoma. NOTE: Staging will be assessed according to standard of care at the treating center.
  • Prior Therapy:
  • Patients should have only previously had a biopsy, and not had prior attempt at tumor resection.
  • Not yet started chemotherapy or radiation therapy OR patient has started chemotherapy or radiation therapy, but an appropriate pre-treatment baseline sample was collected and processed for ctDNA under a local banking study in DFCI Pediatrics and is available to use for this study.
  • Planned to receive chemotherapy as follows:
  • \-- VDC/IE as per COG protocols AEWS0031, AEWS1031 or AEWS1221 (for patients with Ewing sarcoma or PNET); or MAP as per COG protocol AOST0331 (for patients with osteosarcoma).
  • For Part B subjects must meet all of the following eligibility criteria.
  • Age: ≥ 12 months of age at time of study enrollment
  • Diagnosis: Patients with histologic diagnosis (by institutional pathologist) of newly diagnosed Ewing sarcoma or peripheral primitive neuroectodermal tumor (PNET) of bone or soft tissue
  • Prior Therapy:
  • Patients should have had only frontline therapy as per institutional standard, and maintenance therapy if given (no relapse therapy).
  • If frontline systemic therapy already completed (not including maintenance or metastatic site radiation), therapy completed within 6 months of enrollment to Part B.
  • Subjects must have a willing physician provider supporting their participation in Part B.
  • For Part B, providers are eligible to receive the provider survey if they are listed as the primary provider for the patient at the study site.

Exclusion Criteria15

  • Patients with distant metastatic disease.
  • Patients with known Ewing-like sarcoma (e.g., BCOR-CCNB3 or CIC-DUX4 translocated small round cell sarcomas) are not eligible.
  • Patients who are enrolled with an initial diagnosis of Ewing sarcoma and subsequently found to have Ewing-like sarcoma will be replaced. Samples obtained prior to removal from study will be analyzed and reported descriptively. Patients with Ewing-like tumors may continue to provide samples and clinical data until they meet off-study criteria per protocol.
  • Patients weighing \< 5 kg at time of diagnosis
  • Patients with a second malignant neoplasm
  • Patients without detectable tumor at the time of study enrollment (ie, complete tumor resection prior to study enrollment)
  • Patients already receiving tumor-directed therapy at the time of study enrollment except when a pre-treatment baseline sample has already been obtained under a local banking study in DFCI Pediatrics that would be eligible for analysis under this study.
  • Patients with osteosarcoma with a pelvic primary tumor site Pregnancy
  • Patients with known Ewing-like sarcoma (e.g., BCOR-CCNB3 or CIC-DUX4 translocated small round cell sarcomas) are not eligible.
  • Samples obtained prior to removal from study will be analyzed and reported descriptively. Patients with Ewing-like tumors may continue to provide samples and clinical data until they meet off-study criteria per protocol
  • Patients weighing \< 5 kg at time of enrollment
  • Patients diagnosed with relapsed disease and/or having started therapy directed at disease relapse
  • Pregnancy
  • Resides outside of the United States
  • For Part B, providers at non-study centers will not be eligible to receive the provider survey.

Interventions

OTHERFoundationOne Liquid CDx

a FoundationOne liquid biopsy test kit will be sent to the laboratory of the subject's treating center, in addition to the paired cell stabilizing tube from the primary center


Locations(15)

Childrens Hospital Los Angeles

Los Angeles, California, United States

Children's Healthcare of Atlanta

Atlanta, Georgia, United States

Massachusetts General Hospital Cancer Center

Boston, Massachusetts, United States

Boston Children's Hospital

Boston, Massachusetts, United States

Brigham and Women's Hospital

Boston, Massachusetts, United States

Dana Farber Cancer Institute

Boston, Massachusetts, United States

Children's Hospital's and Clinics of Minnesota

Minneapolis, Minnesota, United States

Cincinnati Children's Hospital Medical Center

Cincinnati, Ohio, United States

Nationwide Children's Hospital

Columbus, Ohio, United States

Children's Hospital of Philadelphia

Philadelphia, Pennsylvania, United States

Lifespan / Rhode Island Hospital

Providence, Rhode Island, United States

St. Jude Children's Research Hospital

Memphis, Tennessee, United States

UT Southwestern Medical Center

Dallas, Texas, United States

University of Utah Childrens Medical Center

Salt Lake City, Utah, United States

Seattle Children's Hospital

Seattle, Washington, United States

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NCT06068075


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