RecruitingNCT06189924

Exhaled Breath Condensate Analysis in Mechanically Ventilated Patients

Proteomic Analysis of Exhaled Breath Condensate in Mechanically Ventilated Intensive Care Patients


Sponsor

The Institute of Molecular and Translational Medicine, Czech Republic

Enrollment

200 participants

Start Date

Mar 1, 2026

Study Type

OBSERVATIONAL

Conditions

Summary

Mechanically ventilated intensive care patients will be sampled for a small amount of exhaled breath condensate from the ventilator circuit and for venous blood. Proteomic analysis of the exhaled breath condensate will be performed using mass spectrometry and in the blood sample, corresponding changes in the DNA, RNA, proteins, and metabolites will be studied. Resulting profiles will be correlated with routinely monitored parameters in order to identify patterns corresponding to various pathologies in order to enable their early detection.


Eligibility

Min Age: 18 Years

Inclusion Criteria1

  • Intensive care setting Mechanical ventilation Ventilator/ventilator circuit technically suitable for sampling of exhaled breath condensate

Exclusion Criteria1

  • Mechanical ventilation challenging to the extent that the insertion of the sampling kit into the ventilator circuit would substantially increase the risk for respiratory deterioration

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Interventions

DIAGNOSTIC_TESTProteomic analysis of exhaled breath condensate

Mechanically ventilated intensive care patients will be sampled for exhaled breath condensate from the ventilator circuit.


Locations(2)

Institute of Molecular and Translational Medicine, Palacky University

Olomouc, Czechia

University Hospital Olomouc

Olomouc, Czechia

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NCT06189924


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