RecruitingNCT07168122

Establishment and Clinical Application of Reference Intervals of Salivary Cortisol

Study for Reference Intervals and Optimal Cut-offs for Salivary Cortisol


Sponsor

Shanghai 6th People's Hospital

Enrollment

220 participants

Start Date

Sep 1, 2025

Study Type

OBSERVATIONAL

Conditions

Summary

The goal of this observational study is to establish the normal reference intervals of salivary cortisol and optimal cut-offs for Cushing's sydrome and adrenal insufficiency.


Eligibility

Min Age: 18 YearsMax Age: 80 Years

Inclusion Criteria7

  • Healthy Volunteers:
  • Age ≥ 18 and ≤ 60 years old;
  • Body mass index (BMI) ≥ 18.5 and ≤ 24.9 kg\*m\^-2;
  • No previous history of chronic diseases such as hyperglycemia, hypertension, dyslipidemia, coronary heart disease and stroke;
  • Normal glucose regulation, defined as: fasting blood-glucose \< 5.6 mmol/L, 2-hour blood-glucose after glucose load \< 7.8 mmol/L, and glycated hemoglobin (HbA1c) \< 5.7%.
  • Aged 18 to 80 years old;
  • Patients with suspected Cushing's syndrome or adrenal insufficiency.

Exclusion Criteria12

  • Liver or kidney dysfunction, alanine aminotransferase (ALT) or aspartate aminotransferase (AST) or direct bilirubin higher than 1.5 times the upper limit of normal, or serum creatinine \>115 μmol/L;
  • Pregnancy or lactation women, or people with cancer or mental illness;
  • Factors affecting cortisol levels, including hypothalamus-pituitary-adrenal axis disease, autoimmune disease (systemic lupus erythematosus, rheumatoid arthritis, etc.), diagnosed mental disease, Alzheimer's disease, alcoholism (alcohol \> 60 g/d for male, \>40 g/d for women) and corticosteroid therapy in the past 3 months (females with contraceptives or estrogen);
  • Factors affecting saliva collection, such as serious oral problems (oral ulcers, gingival bleeding);
  • Night shift workers, who are awake from 11:00 PM to 7:00 AM;
  • Acute infection (body temperature ≥ 37.3 ℃ or C-reactive protein \> 50 mg/L).
  • Cases:
  • Corticosteroid therapy within the last 6 weeks;
  • Acute infection;
  • Severe oral conditions;
  • Severe liver and kidney dysfunction;
  • Alcoholism, depression, or other psychiatric disorders.

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Locations(1)

Shanghai Sixth People's Hospital

Shanghai, Shanghai Municipality, China

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NCT07168122


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